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miRNA Research

 

miRNAs are small non-coding RNA molecules that regulate up to 30% of mammalian gene expression. They serve as post-transcriptional regulators and bind to complementary sequences on target messenger RNA transcripts (mRNAs), resulting in translational repression or target degradation. So far, more than 400 miRNAs have been identified in the human genome and many of them are only different in one or a few nucleotides. Dysregulation of miRNA can be associated with many diseases such as cancer; therefore, the research of miRNAs is very important.

Signosis provides a variety of innovative tools for the research of miRNAs including miRBNA arrays for profiling multiple miRNAs simultaneously or monitoring one specific miRNAs.

miRNA Arrays
The miRNA Arrays are used for simultaneously profiling up to 132 miRNAs.

miRNA Northern Blot Assay kits
The assay kits are non-radioactive with all reagents included, and the probes are biotin pre-labeled. Signosis also provides the Highly Sensitive Northern Blot Assay kits, which are 100 times more sensitive than conventional chemiluminescent detection.

small RNA Northern Blot Assay kits

Custom Small RNA Northern Blot Kits detect the expression of small RNA (18nt-200nt). The non-radioactive northern blot assay kits contain everything you need, and the procedure is simpler than Western blotting.

miRNA Luciferase Reporter Vectors
100+ luciferase reporter vectors are available for monitoring different miRNAs in vivo. Signosis also provides custom services for any unlisted miRNA that you might be interested in.

miRNA Real-Time PCR
With the miRNA Real-Time PCR kits, miRNA can be analyzed from total RNA or from cell lysates directly without cDNA conversion.

miRNA Plate Assays

With the Plate Assay kits, the conversion of miRNA into cDNA is not needed. It is 100 times more sensitive than Northern Blot Assay, and the discrimination power of distinguishing the isomers of miRNAs is also higher.

Direct Hybridization Plate Assays
48 miRNAs can be quantitatively analyzed simultaneously via direct hybridization of RNA sample without any addition steps. The procedure is easy, and the conversion of the miRNA into a cDNA is not needed.