Human Apoptosis cDNA Plate Array I

Apoptosis is a cellular process including cytoplasmic condensation and cell shrinkage, chromatin condensation, membrane blebbing, nuclear fragmentation, and the formation of apoptotic bodies, which leads to programmed cell death. During the process, a series of biochemical reactions occur, such as the activation of intracellular proteases and regulation of apoptosis-associated genes. Signosis’ Apoptosis-Associated cDNA Plate Array provides an approach for profiling the expression of apoptosis-regulating genes. Human apoptosis cDNA plate array is a plate-based array for profiling 20+ apoptosis-associated genes.

A. Benefits:

Based on Signosis' proprietary cDNA plate array detection technology, we developed apoptosis cDNA plate array, chich offers following benefits:

• Simple - Do the gene expression profiling as doing ELISA.

• High sensitive detection- Detected mRNA molecules are converted into multiple biotin-labeled cDNAs for sensitive detection.

• Quantitative comparison – The difference of two or more samples in gene expression can be quantitatively analyzed.

B. Principle of the technology

Signosis’ proprietary cDNA plate array is a plate-based hybridization profiling analysis for monitoring the expression of dozens of genes through reverse transcription of mRNA into cDNA. Like array analyses, total RNA is first reverse transcribed into cDNA in the presence of biotin-dUTP in the assay. Targeted genes are then specifically captured onto individual wells on a plate, instead of membranes, through a pre-coated gene-specific oligonucleotide. The captured cDNAs are further detected with streptavidin-HRP. A HRP chemiluminescent substrate is added for measurement. The concentrations of cDNAs are directly proportional to the chemiluminescent intensity of the test sample.