Human Stat3-Regulated cDNA Plate Array

Signal Transducer and Activator of Transcription 3 (Stat3) is a transcription factor that mediates the nuclear action of many different cytokines and growth factors. It plays roles in cell growth and apoptosis. Stat3 also participates in cellular transformation and tumorigenesis. The aberrant activation of Stat3 in tumor cells has been considered as a drug target. Stat3 is activated by tyrosine phosphorylation in response to a wide range of cytokines, growth factors, and hormones. Tyrosine phosphorylation triggers dimerization and nuclear translocation of the protein. Stat3 mediates the expression of its target genes that are involved in a diverse array of biological processes such as oncogenesis, cell growth, and differentiation. Signosis’ Stat3-regulated cDNA plate array allows profiling 20+ Stat3-target genes.

A. Benefits:

Based on Signosis' proprietary cDNA plate array detection technology, we developed Stat3-regulated cDNA plate array, which offers following benefits:

• Simple - Do the gene expression profiling as doing ELISA.

• High sensitive detection- Detected mRNA molecules are converted into multiple biotin-labeled cDNAs for sensitive detection.

• Quantitative comparison – The difference of two or more samples in gene expression can be quantitatively analyzed.

B. Principle of the technology

Signosis’ proprietary cDNA plate array is a plate-based hybridization profiling analysis for monitoring the expression of dozens of genes through reverse transcription of mRNA into cDNA. Like array analyses, total RNA is first reverse transcribed into cDNA in the presence of biotin-dUTP in the assay. Targeted genes are then specifically captured onto individual wells on a plate, instead of membranes, through a pre-coated gene-specific oligonucleotide. The captured cDNAs are further detected with streptavidin-HRP. A HRP chemiluminescent substrate is added for measurement. The concentrations of cDNAs are directly proportional to the chemiluminescent intensity of the test sample.