Human Akt Pathway cDNA Plate Array

Akt is a serine/threonine protein kinase that plays important roles in mediating signals for numerous of cell functions including cell growth, differentiation, cell cycle, transcription, and glucose metabolism. The enzyme is activated by PI3K and activated Akt leads to phosphorylation of a wide range of protein substrates such as GSK-3, IKK-ß, BAD, MDM2, mTOR, CASP9, p21Cip1, p27Kip1. Differential expression of Akt substrates contributes different phenotypic outcomes. Signosis’ Akt pathway-regulated cDNA plate array provides a simple approach to profile the expression of these genes.

A. Benefits:

Based on Signosis' proprietary cDNA plate array detection technology, we developed Akt pathway cDNA plate array, which offers following benefits:

• Simple - Do the gene expression profiling as doing ELISA.

• High sensitive detection- Detected mRNA molecules are converted into multiple biotin-labeled cDNAs for sensitive detection.

• Quantitative comparison – The difference of two or more samples in gene expression can be quantitatively analyzed.

B. Principle of the technology

Signosis’ proprietary cDNA plate array is a plate-based hybridization profiling analysis for monitoring the expression of dozens of genes through reverse transcription of mRNA into cDNA. Like array analyses, total RNA is first reverse transcribed into cDNA in the presence of biotin-dUTP in the assay. Targeted genes are then specifically captured onto individual wells on a plate, instead of membranes, through a pre-coated gene-specific oligonucleotide. The captured cDNAs are further detected with streptavidin-HRP. A HRP chemiluminescent substrate is added for measurement. The concentrations of cDNAs are directly proportional to the chemiluminescent intensity of the test sample.