Human Wnt/b-Catenin Regulated cDNA Plate Array

The Wnt signaling pathway is important to both embryonic development and tumorigenesis. ß-Catenin, the central component of the pathway, functions as a cofactor of the TCF/LEF family of transcription factors and activates transcription of Wnt target genes by binding to the promoters of downstream target genes involved in cell proliferation, survival, and migration. A number of proteins including the tumor suppressor APC and Axin are involved in the regulation of the Wnt signaling pathway. Signosis’ Wnt/ß-Catenin signaling pathway cDNA plate array allows measuring the expression of 21 genes that participate in the pathway at a same time. The difference of these genes in expression among different samples can be determined and compared by using the assay kit. Signosis’ Wnt/b-Catenin cDNA Plate Array allows profiling 20+ related genes.

A. Benefits:

Based on Signosis' proprietary cDNA plate array detection technology, we developed human Wnt/b-Catenin regulated cDNA plate array, which offers following benefits:

• Simple - Do the gene expression profiling as doing ELISA.

• High sensitive detection- Detected mRNA molecules are converted into multiple biotin-labeled cDNAs for sensitive detection.

• Quantitative comparison – The difference of two or more samples in gene expression can be quantitatively analyzed.

B. Principle of the technology

Signosis’ proprietary cDNA plate array is a plate-based hybridization profiling analysis for monitoring the expression of dozens of genes through reverse transcription of mRNA into cDNA. Like array analyses, total RNA is first reverse transcribed into cDNA in the presence of biotin-dUTP in the assay. Targeted genes are then specifically captured onto individual wells on a plate, instead of membranes, through a pre-coated gene-specific oligonucleotide. The captured cDNAs are further detected with streptavidin-HRP. A HRP chemiluminescent substrate is added for measurement. The concentrations of cDNAs are directly proportional to the chemiluminescent intensity of the test sample.