Human Stem Cell Marker cDNA Plate Array

Stem cells have important characteristics distinguishing from other cell types. They are unspecialized cells capable of renewing themselves through cell division and differentiating into a variety of cell lineages. In addition, they serve as an internal repair system, replenishing specialized cells, in order to maintain the normal turnover of regenerative organs. Identification of a set of stem cell –specific genes can function as stem cell molecular biomarkers to facilitate stem cell research. Signosis’ Stem Cell Marker cDNA Plate Array allows profiling 30+ related genes.

A. Benefits:

Based on Signosis' proprietary cDNA plate array detection technology, we developed human stem cell cDNA plate array, which offers following benefits:

• Simple - Do the gene expression profiling as doing ELISA.

• High sensitive detection- Detected mRNA molecules are converted into multiple biotin-labeled cDNAs for sensitive detection.

• Quantitative comparison – The difference of two or more samples in gene expression can be quantitatively analyzed.

B. Principle of the technology

Signosis’ proprietary cDNA plate array is a plate-based hybridization profiling analysis for monitoring the expression of dozens of genes through reverse transcription of mRNA into cDNA. Like array analyses, total RNA is first reverse transcribed into cDNA in the presence of biotin-dUTP in the assay. Targeted genes are then specifically captured onto individual wells on a plate, instead of membranes, through a pre-coated gene-specific oligonucleotide. The captured cDNAs are further detected with streptavidin-HRP. A HRP chemiluminescent substrate is added for measurement. The concentrations of cDNAs are directly proportional to the chemiluminescent intensity of the test sample.