Human T Cell Receptor Signaling cDNA Plate Array

T-Cells are a subset of lymphocytes that play a key role in the immune response. T-Cell Receptor (TCR) is a complex of integral membrane proteins that participates in the activation of T-Cells in response to antigen. This activation involves subunits of the receptor complex as well as other membrane-associated molecules that couple the extracellular liganding event to downstream signaling pathways such as the increase of inositol phosphates, the elevation of intracellular calcium levels and protein phosphorylation. TCR activation promotes a number of signaling cascades that ultimately determine cell fate through regulating cytokine production, cell survival, proliferation, and differentiation. Signosis’ Human T Cell Receptor Signaling cDNA Plate Array allows profiling 20+ related genes.

A. Benefits:

Based on Signosis' proprietary cDNA plate array detection technology, we developed human T cell receptor signalingcDNA plate array, which offers following benefits:

• Simple - Do the gene expression profiling as doing ELISA.

• High sensitive detection- Detected mRNA molecules are converted into multiple biotin-labeled cDNAs for sensitive detection.

• Quantitative comparison – The difference of two or more samples in gene expression can be quantitatively analyzed.

B. Principle of the technology

Signosis’ proprietary cDNA plate array is a plate-based hybridization profiling analysis for monitoring the expression of dozens of genes through reverse transcription of mRNA into cDNA. Like array analyses, total RNA is first reverse transcribed into cDNA in the presence of biotin-dUTP in the assay. Targeted genes are then specifically captured onto individual wells on a plate, instead of membranes, through a pre-coated gene-specific oligonucleotide. The captured cDNAs are further detected with streptavidin-HRP. A HRP chemiluminescent substrate is added for measurement. The concentrations of cDNAs are directly proportional to the chemiluminescent intensity of the test sample.