Human PPARgamma-Regulated cDNA Plate Array

The peroxisome-proliferator activated receptor gamma (PPARr) is a member of the nuclear receptor superfamily of transcription factors. PPARr is important to a variety of biological processes, such as adipocyte differentiation, glucose homeostasis, lipid trafficking as well as vascular function and hypertension. PPARr exerts its effects by regulating target gene transcription in a ligand-dependent manner. Upon activation, the PPARr-RXR heterodimer binds to specific DNA sequences on the target gene promoter and leads to transcriptional regulation. Signosis developed a plate-based array for profiling 20+ PPARr-regulated genes.

A. Benefits:

Based on Signosis' proprietary cDNA plate array detection technology, we developed PPARγ regulated cDNA plate array, which offers following benefits:

• Simple - Do the gene expression profiling as doing ELISA.

• High sensitive detection- Detected mRNA molecules are converted into multiple biotin-labeled cDNAs for sensitive detection.

• Quantitative comparison – The difference of two or more samples in gene expression can be quantitatively analyzed.

B. Principle of the technology

Signosis’ proprietary cDNA plate array is a plate-based hybridization profiling analysis for monitoring the expression of dozens of genes through reverse transcription of mRNA into cDNA. Like array analyses, total RNA is first reverse transcribed into cDNA in the presence of biotin-dUTP in the assay. Targeted genes are then specifically captured onto individual wells on a plate, instead of membranes, through a pre-coated gene-specific oligonucleotide. The captured cDNAs are further detected with streptavidin-HRP. A HRP chemiluminescent substrate is added for measurement. The concentrations of cDNAs are directly proportional to the chemiluminescent intensity of the test sample.