Single Cell RT-PCR Assay Kit

Gene expression is regulated at the level of individual cells. It is desired to have methods capable of analyzing the gene expression pattern of individual cells such as stem cells, neurons, developmental tissues, and laser capture microssections. Signosis has developed a sensitive single cell RT-PCR directly in cell lysates without RNA preparation. Therefore, a small number of cells can be used for RT-PCR analysis. Signosis now offers the optimized buffers for subsequent RT-PCR followed cell lysis with CL™ cell lysis buffer. All of reagents for cDNA synthesis and PCR amplification in addition to CL™ cell lysis buffer are included in the kit.

A. Benefits:

Signosis’ single cell RT-PCR assay kit offers following benefits:

  • Convenient - All reagents, from cell lysis, reverse transcription to PCR, are included in one kit.

  • No RNA preparation -Cell lysates can be used directly in reverse transcription.

  • Robust -Full length cDNA can be obtained with the kit.

B. Principle of the technology

The buffers are optimized for cell lysate preparation and RT-PCR.

Figure 1
Diagram

Figure 2. Single Cell RT-PCR Assay Kit. A: The indicated cells were lysed with cell lysis buffer from Signosis and competitor respectively, and subjected to RT-PCR for ß-actin with 30 cycles. B: Testing no genomic DNA contamination. Lane1. ß-actin was amplified with 36 PCR cycles directly from cell lysate without reverse transcription (RT). Lane2. ß-actin was amplified with 36 PCR cycles directly from cDNA transcribed from cell lysate.

 

 Product Size Cat. Price
Single Cell RT-PCR Assay Kit 10 CL-0002 $245
  • Single Cell RT-PCR User Manual (PDF)

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