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Human Adiponectin ELISAAdiponectin, also called GBP-28, apM1, AdipoQ and Acrp30, is an adipose tissue-specific protein that circulates in human plasma at high levels. Plasma levels of adiponectin has been reported to be significantly reduced in obese/diabetic mice and humans. It is also decreased in patients with cardiovascular diseases, hypertension or metabolic syndrome. On the contrary, a number of other human diseases such as chronic renal failure, type 1 diabetes and anorexia nervosa are associated with increased plasma adiponectin levels. Plasma adiponectin correlates negatively with glucose, insulin, triglyceride levels and body mass index, and positively with high-density lipoprotein-cholesterol levels and insulin-stimulated glucose disposal. A. Benefits:
B. Principle of the technology Adiponectin ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay utilizes a mouse anti-human adiponectin antibody for immobilization on the microtiter wells and a biotinated mouse anti-human adiponectin antibody along with streptavidin conjugated to horseradish peroxidase (HRP) for detection. The test sample is allowed to react simultaneously with the two antibodies, resulting in the adiponectin molecules being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The concentration of adiponectin is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm. Figure 1 Figure 2
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