Human IGF-1 ELISA

Insulin-like growth factor-I (IGF-I) acts as an important mediator between growth hormone and growth throughout fetal and childhood development. More circumstantial evidence indicates the association of IGF-I to the risk of cancer. High concentrations of IGF-I has been shown to be an increased risk of colorectal cancer and breast cancer in some studies and less consistently with prostate, thyroid, and haematological malignancies. IGF-I is a potent mitogen and important stimulus for adipocyte differentiation. IGF-I can reduce hyperglycemia in patients with severe insulin resistance by direct effects mediated via the IGF-I receptor. IGF-I infusion lowers insulin and lipid levels in healthy humans and reduces plasma leptin concentrations in rats, suggesting that IGF-I may reduce the degree of insulin resistance in type 2 diabetes, obesity, and hyperlipidemia.

A. Benefits:

• Cost-effective analysis - High Quality with the most competitive price.

• Efficient and flexible - Multiple samples can be analyzed simultaneously with a flexible number of assay wells.

• Specific- A pair of highly selective antibodies specifically against human IGF-1.

• Simple procedure All in one system with a pre-coated 96-well plate.

B. Principle of the technology

IGF-I ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay utilizes a mouse anti-human IGF-I antibody for immobilization on the microtiter wells and goat anti-human IGF-I antibodies along with streptavidin conjugated to horseradish peroxidase (HRP) for detection. The test sample is allowed to react simultaneously with the two antibodies, resulting in the IGF-I molecules being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The concentration of IGF-I is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.

Home |  Product List |  Literature |  Order |  Contact Us