Human IL-6 ELISA

Interleukin-6 (IL-6) is a multifunctional cytokine that regulates the immune response, hematopoiesis, the acute phase response, and inflammation. Deregulation of IL-6 production is implicated in the pathology of several disease processes. Its levels are observed in several diseases, including rheumatoid arthritis (RA). IL-6 plays roles in both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response. In addition, like TNFa, IL-6 is another adipocyte secretory product that may be involved in insulin resistance. IL-6 is a cytokine secreted by many cells, including adipocytes and adipose stromal cells. IL-6 secretion is increased in the adipocytes of obese subjects and may be important either as a circulating hormone or as a local regulator of insulin action. IL-6 has been implicated in the development of insulin resistance and type 2 diabetes in obese individuals. Like TNF, IL-6 inhibits the expression of LPL, but, unlike TNF, IL-6 does not stimulate lipolysis.

A. Benefits:

• Cost-effective analysis - High Quality with the most competitive price.

• Efficient and flexible - Multiple samples can be analyzed simultaneously with a flexible number of assay wells.

• Specific- A pair of highly selective antibodies specifically against human IL-6.

• Simple procedure All in one system with a pre-coated 96-well plate.

B. Principle of the technology

IL-6 ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay utilizes a mouse anti-human IL-6 antibody for immobilization on the microtiter wells and goat anti-human IL-6 antibodies along with streptavidin conjugated to horseradish peroxidase (HRP) for detection. The test sample is allowed to react simultaneously with the two antibodies, resulting in the IL-6 molecules being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The concentration of IL-6 is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.

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