Human TGF-ß1 ELISA

The transforming growth factor beta 1 (TGF-ß1) gene codes a multifunctional cytokine that controls proliferation, differentiation, and other functions in many cell types, including cancer cells, the surrounding stromal cells, immune cells, endothelial and smooth-muscle cells. It causes immunosuppression and angiogenesis, which makes the cancer more invasive. TGF-ß also converts effector T-cells, which normally attack cancer with an inflammatory (immune) reaction, into regulatory (suppressor) T-cells, which turn off the inflammatory reaction. TGF-ß induces apoptosis in numerous cell types. TGF-ß can act on adipocyte precursor cells. TGF- ß1 has been shown to be a potent inhibitor of the differentiation of adipogenic cell lines. In addition, a differentiation-defective, insulin-independent cell linederived from the adipogenic cell line 1246 produces in its conditional medium a TGF- ß1-like polypeptide which could modulate the cell ability to differentiate in an autocrine fashion. Increased TGF-b1 expression was associated with BMI and abdominal adipose tissue in morbid obesity.

A. Benefits:

• Cost-effective analysis - High Quality with the most competitive price.

• Efficient and flexible - Multiple samples can be analyzed simultaneously with a flexible number of assay wells.

• Specific- A pair of highly selective antibodies specifically against human TGF-ß1.

• Simple procedure All in one system with a pre-coated 96-well plate.

B. Principle of the technology

TGF-ß1 ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay utilizes a mouse anti-human TGF-ß1 antibody for immobilization on the microtiter wells and chicken anti-human TGF-ß1 antibodies along with streptavidin conjugated to horseradish peroxidase (HRP) for detection. The test sample is allowed to react simultaneously with the two antibodies, resulting in the TGF-ß1 molecules being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The concentration of TGF-ß1 is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.

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