Human FGFb ELISA

Fibroblast growth factor (FGF)-2, also known as basic FGF (FGFb), is a powerful stimulator of angiogenesis in vivo, in addition to being a pleiotropic regulator of the proliferation, migration, differentiation, and survival of many cell types in vitro, including endothelial cells, smooth muscle cells, and pericytes. It is capable of inducing an angiogenic response both in vivo and in vitro. Both basic and acidic FGFs, a heparin-binding family of structurally related cytokines, are widely expressed during embryonic development and normal wound healing, as well as in such angioproliferative diseases as cancer and diabetes.

A. Benefits:

• Cost-effective analysis - High Quality with the most competitive price.

• Efficient and flexible - Multiple samples can be analyzed simultaneously with a flexible number of assay wells.

• Specific- A pair of highly selective antibodies specifically against human FGFb.

• Simple procedure All in one system with a pre-coated 96-well plate.

B. Principle of the technology

FGFb ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay utilizes a mouse anti-human FGFb antibody for immobilization on the microtiter wells and mouse anti-human FGFb antibody along with streptavidin conjugated to horseradish peroxidase (HRP) for detection. The test sample is allowed to react simultaneously with the two antibodies, resulting in the FGFb molecules being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The concentration of FGFb is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.

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