Human EGF ELISA

Epidermal growth factor (EGF) is a strong mitogen for many cells, such as fibroblasts. EGF also stimulates the proliferation of embryonic cells. The proliferation of some cell lines has been shown to be inhibited by EGF. EGF acts as a differentiation factor for some cell types. To a limited extent EGF also augments angiogenesis because it is mitogenic for endothelial cells. The mitogenic activity of EGF for endothelial cells can be potentiated by thrombin. EGF is a strong chemoattractant for fibroblasts and epithelial cells. EGF alone and also in combination with other cytokines is an important factor mediating wound healing processes.

A. Benefits:

• Cost-effective analysis - High Quality with the most competitive price.

• Efficient and flexible - Multiple samples can be analyzed simultaneously with a flexible number of assay wells.

• Specific- A pair of highly selective antibodies specifically against human EGF.

• Simple procedure All in one system with a pre-coated 96-well plate.

B. Principle of the technology

EGF ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay utilizes a mouse anti-human EGF antibody for immobilization on the microtiter wells and goat anti-human EGF antibody along with streptavidin conjugated to horseradish peroxidase (HRP) for detection. The test sample is allowed to react simultaneously with the two antibodies, resulting in the EGF molecules being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The concentration of EGF is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.

Home |  Product List |  Literature |  Order |  Contact Us