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Human PDGF-BB ELISAPlatelet-derived growth factor (PDGF) is a growth factor that is important to embryonic development, cell proliferation, and cell migration, particularly, in blood vessel formation (angiogenesis). Like VEGF, PDGF is able to independently initiate angiogenesis and mediate blood vessel growth and behavior. The protein is a dimeric glycoprotein composed of two A (-AA) or two B (-BB) chains or a combination of the two (-AB). It has been shown that the cis oncogene is derived from the PDGF B-chain gene. It binds to PDGF receptors, which activate signal transduction pathways such as the PI3K pathway, and subsequently regulate downstream gene expression and the cell cycle. PDGF has also been linked to several diseases such as atherosclerosis, fibrosis and malignant diseases. A. Benefits:
B. Principle of the technology PDGF ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay utilizes rabbit anti-human PDGF antibodies for immobilization on the microtiter wells and rabbit anti-human PDGF antibodies along with streptavidin conjugated to horseradish peroxidase (HRP) for detection. The test sample is allowed to react simultaneously with the two antibodies, resulting in the PDGF molecules being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The concentration of PDGF is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm. Figure 1 Figure 2
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