Human b-NGF ELISA

Nerve growth factor (NGF) is a small secreted protein which induces the differentiation and survival of particular target neurons (nerve cells). NGF has recently been identified as a novel angiogenic molecule, which exerts a variety of effects in the cardiovascular system and on endothelial cells. NGF may contribute to maintenance, survival, and function of endothelial cells by autocrine and/or paracrine mechanisms. Beta-NGF (bNGF) is structurally related to BDNF, NT-3 and NT-4. NGF is a potent neurotrophic factor that signals through its receptor beta-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Beta-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival.

A. Benefits:

• Cost-effective analysis - High Quality with the most competitive price.

• Efficient and flexible - Multiple samples can be analyzed simultaneously with a flexible number of assay wells.

• Specific- A pair of highly selective antibodies specifically against human NGF.

• Simple procedure All in one system with a pre-coated 96-well plate.

B. Principle of the technology

Beta-NGF ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay utilizes rabbit anti-human bNGF antibodies for immobilization on the microtiter wells and rabbit anti-human bNGF antibodies along with streptavidin conjugated to horseradish peroxidase (HRP) for detection. The test sample is allowed to react simultaneously with the two antibodies, resulting in the bNGF molecules being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The concentration of bNGF is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.

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