Human IL-8 ELISA

Interleukin-8 (IL-8) is a chemokine produced by macrophages and other cell types such as epithelial cells. IL-8 targets a relative wide range of cells such as endothelial cells, macrophages, mast cells, keratinocytes, but neutrophil granulocytes are the primary target cells. IL-8 is one of the major mediators of the inflammatory response by attracting neutrophils at the site of inflammation.. This chemokine is also a potent angiogenic factor.

A. Benefits:

• Cost-effective analysis - High Quality with the most competitive price.

• Efficient and flexible - Multiple samples can be analyzed simultaneously with a flexible number of assay wells.

• Specific- A pair of highly selective antibodies specifically against human IL-8.

• Simple procedure All in one system with a pre-coated 96-well plate.

B. Principle of the technology

IL-8 ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay utilizes goat anti-human IL-8 for immobilization on the microtiter wells and biotinated goat anti-human IL-8 antibodies along with streptavidin conjugated to horseradish peroxidase (HRP) for detection. The test sample is allowed to react simultaneously with the two antibodies, resulting in the IL-8 molecules being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The concentration of IL-8 is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.

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