Human IP-10 ELISA

IFN-gamma-inducible protein (IP-10) is a member of the chemokine family of cytokines and is induced in a variety of cells in response to interferon gamma and lipopolysaccharide. It is secreted by a number of cells including monocytes, endothelial cells and fibroblasts. IP-10 plays several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation. several cell types in response to IFN-?. IP-10 acts as potent inhibitors of angiogenesis in vivo.

A. Benefits:

• Cost-effective analysis - High Quality with the most competitive price.

• Efficient and flexible - Multiple samples can be analyzed simultaneously with a flexible number of assay wells.

• Specific- A pair of highly selective antibodies specifically against human IP-10.

• Simple procedure All in one system with a pre-coated 96-well plate.

B. Principle of the technology

IP-10 ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay utilizes rabbit anti-human IP-10 for immobilization on the microtiter wells and biotinated rabbit anti-human IP-10 antibodies along with streptavidin conjugated to horseradish peroxidase (HRP) for detection. The test sample is allowed to react simultaneously with the two antibodies, resulting in the IP-10 molecules being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The concentration of IP-10 is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.

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