Mouse Inflammation ELISA Strip for profiling 8 Cytokines

Cytokines are extracellular signaling proteins produced by different cell types that act on target cells to modulate diverse cellular functions, such as recruiting specific cell types to the site of inflammation, increasing the activation and survival of immune cells, or suppressing cellular activity. Inflammation is the response of tissue to injury. During both acute and chronic inflammatory processes, a variety of soluble factors are involved in the cellular infiltrate, the cellular activation, and the systemic responses to inflammation. Cytokines are major determinants of inflammatory responses. Most cytokines are multifunctional molecules that elicit their effects locally or systemically in an autocrine or paracrine manner. Cytokines are involved in extensive networks that involve synergistic as well as antagonistic interactions and exhibit both negative and positive regulatory effects on various target cells. Therefore, profiling the expression pattern of cytokines provides a valuable insight to the underlying immunological mechanisms. Signosis’ Mouse Inflammation ELISA Strip allows quantitatively profiling and measuring 8 cytokines; IL-1a, IL-1b, G-CSF, GM-CSF, MCP-1, MIP-1a, SCF, and Rantes.

A. Benefits:

• Multiplex - A single ELISA strip allows the measurement of 8 inflammation cytokines.

• Flexible - The comparison can be made from 2 samples to 12 samples with a single plate.

• Quantitative measurment of differences - Unlike membrane-based array assays, the differences of individual proteins among samples can be quantitatively compared. In addition, EA-1052 provides protein standards for making standard curves.

• No need of capital equipment - Unlike beads-based assays, no capital equipment is required.

• Simple procedure -All in one system with a pre-coated 12 X 8 well plate.

B. Principle of the technology

In each well of the strip, a primary antibody against a specific inflammation cytokine is coated and 8 wells of the strip are coated with 8 different antibodies. Therefore, total 8 wells of a strip allow measurement of 8 different cytokines. The test sample is allowed to react simultaneously with pairs of two antibodies, resulting in the inflammation cytokines being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The concentrations of the inflammation cytokines are directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.

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