Human Growth Factor ELISA Strip for Profiling 8 Proteins

Growth factors are a family of proteins capable of stimulating cellular growth, proliferation and cellular differentiation. They typically act as signaling molecules by binding to receptors on the cell surface. Many growth factors are quite versatile, stimulating cellular division in numerous different cell types; while others are specific to a particular cell-type. Multiple growth factors often together participate in cellular functions. For example, VEGF, FGF, and EGF stimulate angiogenesis. Therefore, profiling the expression pattern of growth factors provides a valuable insight to the biological mechanisms underlying cellular functions. Signosis’ Growth Factor ELISA Strip allows quantitatively profiling and measuring 8 proteins; VEGF, EGF, TNFa, IFNr, PDGF-BB, PIGF, b-NGF, and SCF.

A. Benefits:

• Multiplex - A single ELISA strip allows the measurement of 8 growth factors.

• Flexible - The comparison can be made from 2 samples to 12 samples with a single plate.

• Quantitative measurment of differences - Unlike membrane-based array assays, the differences of individual proteins among samples can be quantitatively compared. In addition, EA-1062 provides protein standards for making standard curves.

• No need of capital equipment - Unlike beads-based assays, no capital equipment is required.

• Simple procedure -All in one system with a pre-coated 12 X 8 well plate.

B. Principle of the technology

In each well of the strip, a primary antibody against a specific growth factor is coated and 8 wells of the strip are coated with 8 different antibodies. Therefore, total 8 wells of a strip allow measurement of 8 different growth factors. The test sample is allowed to react simultaneously with pairs of two antibodies, resulting in the growth factors being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The concentrations of the growth factors are directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.

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