Mouse Cytokine ELISA Strip I for Profiling 8 Proteins

Cytokines are essential molecules play crucial roles in many biological functions, including viral infection, inflammation, immunity, and hematopoiesis. Cytokines are produced by a variety of cell types in response to different stimuli. In addition, the expression of cytokine genes appears to be regulated by complex mechanism. Expression of one cytokine gene could be regulated by other cytokines. Dysregulation of cytokine gene expression may be caused by chromosomal alterations or by infection of viruses that induce activation or inactivation of the expression machinery. Therefore, profiling of these cytokines is critical to understanding these biological functions. Signosis’ Mouse Cytokine ELISA Strip I Profiling Assay allows simultaneously profiling 8 cytokines; Leptin, TNFa, IGF-1, IL-6, VEGF, IL-1a, IL-1ß, and GCSF. Each well of the strip is coated with a primary antibody against a specific cytokine and total 8 wells of a strip target 8 different cytokines. The difference of these proteins between two samples can be determined through data comparison.

A. Benefits:

• Multiplex - A single ELISA strip allows the measurement of 8 cytokines.

• Flexible - The comparison can be made from 2 samples to 12 samples with a single plate.

• Quantitative measurment of differences - Unlike membrane-based array assays, the differences of individual proteins among samples can be quantitatively compared. In addition, EA-1092 provides protein standards for making standard curves.

• No need of capital equipment - Unlike beads-based assays, no capital equipment is required.

• Simple procedure -All in one system with a pre-coated 12 X 8 well plate.

B. Principle of the technology

In each well of the strip, a primary antibody against a specific cytokine is coated and 8 wells of the strip are coated with 8 different antibodies. Therefore, total 8 wells of a strip allow measurement of 8 different cytokines. The test sample is allowed to react simultaneously with pairs of two antibodies, resulting in the cytokines being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The concentrations of the cytokines are directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.

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