Human SATB1 ELISA

SATB1 is a nuclear protein which plays a crucial role in metastasis of breast cancer (1). It promotes tumor growth and metastasis by changing the expression of hundreds of genes, affecting cell adhesion, cell signaling, cell-cycle regulation, and other functions, including the epidermal growth factor gene Her2. SATB1 is not expressed in all cells. Only the metastatic cells expressed SATB1, with the most aggressive breast cancer cells showing the highest levels of the protein. When SATB1 is detected in a breast tumor, the cancer is highly likely to progress or recur. Studies with human primary breast cancer tissue samples for which clinical follow-up studies indicated that the highest levels of SATB1 were in samples from patients whose survival times had been shortest; patients whose tumor samples had no SATB1 expression generally had longer survival times. In addition, SATB1's ability to regulate gene expression was identified as critical to T-cell development. Studies revealed that SATB1 interacts with HDAC1 or PCAF, is regulated by phosphorylation and cleaved by Caspase 6. It expresses high in Jurkat and low in HeLa cells.

A. Benefits:

  • Cost-effective analysis - High Quality with the most competitive price.

  • Efficient and flexible - Multiple samples can be analyzed simultaneously with a flexible number of assay wells.

  • Specific- A pair of highly selective molecules specifically bound to human SATB1.

  • Simple procedure - All in one system with a pre-coated 96-well plate.

B. Principle of the technology

SATB1 ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay utilizes biotin labeled DNA binding sequence of SATB1 for immobilization of SATB!/DNA complex onto the streptavidin-coated microtiter wells, which is detected by an anti-SATB1 antibody and anti-mouse IgG conjugated to horseradish peroxidase (HRP). After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The concentration of adiponectin is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.

Figure 1
Diagram

Figure 2
Figure 2

Figure 2. ELISA (top) and gel shift (bottom) of SATB1 in Jurkat and HeLa cells.

 

 Product Size Cat. Price
Human SATB1 ELISA 96 EA-2001 $459
  • Human SATB1 ELISA User Manual (PDF)
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