TF Activation Profiling Plate Array I

Transcription factors (TFs) are a group of cellular proteins that play essential roles in regulating gene expression. They act as a sensor to monitor cellular change and convert the signals into gene expression. Often, a specific cellular signal pathway can activate multiple TFs and the expression of a specific gene is under the control of multiple TFs. Hence, monitoring the activation of multiple TFs simultaneously is critical to understanding the molecular mechanism of cellular regulation underlying cell signaling and gene expression. Signosis’ TF activation profiling plate array I is used for monitoring 48 different TFs including NFkB, AP1, HIF1, and p53.

A. Benefits:

Based on its proprietary technology, Signosis developed TF activation profiling plate array, which offers following benefits:

• Multiplex - A single assay allows the measurement of the activities of 48 TFs .

• Quantitative comparison - The difference of two samples can be quantitatively analyzed and compared.

• Simple procedures - Probe incubation, spin column separation, plate hybridization, and HRP detection.

• No special requirement -Capital instrument such as Luminex is not needed.

B. Principle of the technology

Signosis’ TF activation profiling plate array is used for monitoring the activation of multiple TFs simultaneously. In the technology, a series of biotin-labeled probes are made based on the consensus sequences of TF DNA-binding sites. When the probe mix incubate nuclear extracts, individual probes will find its corresponding TF and form TF/probe complexes, which can be easily separated from free probes through a simple spin column purification. The bound probes are detached from the complex and analyzed through hybridization with a plate; each well is specifically pre-coated with complementary sequences of the probes.

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