TF Gel Shift Assays

Transcription factors (TFs) are a group of cellular proteins that control gene expression. Once a TF is activated, it binds to its corresponding cis-element within promoter regions and mediates its target gene expression. Gel shift assay is a commonly used method for monitoring the activation of a TF in vitro. Signosis’ TF Gel Shift Assays are a series of pre-made kits to taregt 50+ TFs.

A. Benefits:

Signosis' TF Gel Shift Assays offer following benefits:

• No isotope is required - - It is a chemiluminescent-based detection.

• No probe preparation - Biotin pre-labeled probes are available.

• Simple procedure – The assay is simple and straightforward.

B. Principle of the technology

When one TF is activated, it binds to a biotin-labeled probe, which is designed based on the consensus sequence of the TF binding. When the formed complex is subject to electrophoresis, it runs slower than the free probe, which can be easily distinguished. Two or more samples can be compared by the shift assays and the difference can be determined by the density of the shifted band density.

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