TF Luciferase Reporter Vectors

pTF-Luc reporter vectors are a series of firefly luciferase-based reporter constructs for quantitative measurement of the activities of transcription factors (TFs) in cells. Each vector contains a cis-element (DNA binding sequence), a minimal promoter, and firefly luciferase gene. When a TF is activated, it binds to cis-element and results in induction of luciferase gene expression. Therefore, luciferase activity represents the activity of the TF.

A. Benefits:

TF Luciferase Reporter Vectors offer the following benefits:

• Functional assay - It provides functional assays to monitor the activities of TFs in cells.

• Quantitative analysis - Activities of TFs are measured through repression of firefly luciferase gene expression.

• Stable cell lines - The vectors can be used for establishing stable cells expressing the reporters.

B. Principle of the technology

TF targets its binding site at upstream of TATA promoter to induce the expression of luciferase gene. It results in induction of luciferase enzyme activity. Through comparison of luciferase activity in two samples, up- or down-reguated TF can be quantitatively measured.

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