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siRNA Plate AssaySmall interfering RNAs (siRNAs) are double-stranded RNA molecules with approximately 20-25 nucleotides in length. Since synthetic siRNAs were shown to be able to induce RNAi in mammalian cells in 2001, siRNAs have drawn lots of attention in biomedical research and drug development. They have been widely used for silencing gene expression. To monitor the delivery and expression of siRNA is important to ensuring the success of the knockdown. Northern blot is a common approach to analyze the molecules. However, the assay is not sensitive and the procedure tedious. Signosis has developed a high sensitive siRNA plate assay. It is 1000 times more sensitive than Northern blot. In addition, the assay is just simple incubations, as simple as ELISA. More importantly, a large of samples can be analyzed simultaneously. Based on the sequence of a specific siRNA provided by you, Signosis will design and synthesize a set of oligos and provide an assay kit back to you. A. Benefits: Signosis' siRNA plate arrays offer following benefits:
B. Principle of the technology In the proprietary siRNA plate assay, one siRNA molecule is flanked by a capture oligo and a biotinated detection oligo through two bridge oligos. One of the bridge oligos is partially hybridized with the siRNA molecule and the capture oligo and another one with the siRNA and the detection oligo. The hybrid is captured onto plate through hybridization with an immobilized oligo and detected by a streptavidin-HRP conjugate and chemiluminecscent substrate. This hybrid structure is sensitive to the sequence of the siRNA molecule. One nucleotide difference can prevent the formation of the hybrid and therefore siRNA isoform can be differentiated, which normally is hard to do with Northern blot. In addition, the sensitivity of the assay is higher than siRNA Northern blot assay. Figure 1 <
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