Cancer miR-21 Northern Blot Assay Kit

miR-21 is overexpressed in a wide variety of cancers. It is believed to be an oncogene by downregulating the expression of many tumor suppressors, including programmed cell death 4 (PDCD4), tumor suppressor Pdcd4, the PTEN tumor suppressor gene, and the tumor suppressor gene tropomyosin 1 (TPM1). miR-21 also found to be an important regulator of cancer-cell survival. Differently from the survival pathway mediated by IL-6/Stat3 activation through expression of antiapoptotic proteins, miR-21 is upregulated by Stat3, which mediates the suppression of apoptosis possibly through the inhibition of TPM1 and/or other proteins.

A. Benefits:

MiRNA Northern blot assay kits offer the following benefits:

• No isotope is required - - It is a chemiluminescent-based detection.

• No probe preparation - Biotin pre-labeled probes are available.

• All components in one kit - Gel, membrane, and solutions for RNA transfer, hybridization, and detection along with miR-21 probe are provided in the kits.

• Simple procedure – The assay is simple and straightforward.

B. Principle of the technology

RNA samples are separated through gel electrophoresis and transferred onto a membrane. Expression of a specific miRNA is detected with a biotin-labeled probe.