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Cell Viability Cytotoxicity (CVC) Reagent

 
Product   Principle   Data   Literature   Price/Order   Message
 

Sample type: Adherent cells, Suspension cells

Species reactivity: Mammalian

Assay time: 30-60m

Kit size-: 100, 500 Tests

Storage Conditions: 2-4°C

 

Overview

Cell Viability/Cytotoxicity (CVC) Reagent is a colorimetric WST-8 based assay that provides a simple and accurate method to measure cell proliferation. This is a tetrazolium reduction assays based on the cleavage of the tetrazolium salt WST-8 to water-soluble, orange formazan dye by cellular mitochondrial dehydrogenases (Fig.1). Intensity of dye is a direct proportion of increased activity of the mitochondrial dehydrogenases and therefore number of viable cells which can be quantified by measuring the absorbance at 450 nm.

Ready to use CVC Reagent allow for rapid, sensitive and reliable method for measuring cell viability/cytotoxicity directly from a 96-well plate without any additional processing.

 

 


 

Figure 1.  WST-8 Assay Mechanism

 

 

Application

CVC Reagent is suitable for measuring cell viability and proliferation in response to cell growth and signaling factors. It can also be used to measure cytotoxicity of cytotoxic compounds like anticancer drugs. As shown in the graph below:


 Dose-dependent growth inhibition of BaF3 cells harboring EGFR Del19 (EL-011), EGFR Ins20 (EL-009) and representative Erlotinib resistant cell line, EGFR Del19-T790M (EL-014). The cells were treated with the indicated dose of erlotinib for 24 hours and cells viability was measured using WST-8 assay. EGFR Del19 stably expressing BaF3 cells (EL-011) remains 14-fold more sensitive to TKI erlotinib (IC50=0.34) than its resistance derivative EGFR Del19-T790M stably expressing BaF3 cells (EL-014) (IC50=4.9).

 

 

Cell Viability Cytotoxicity (CVC) Reagent 1ml


 Product Name Amount Catalog #
 Cell Viability/Cytotoxicity (CVC) Reagent 1 ml CVC001

 

 

 




Cell Viability Cytotoxicity (CVC) Reagent 5ml


 Product Name Amount Catalog #
 Cell Viability/Cytotoxicity (CVC) Reagent 5 ml CVC005