PRODUCTS   METHODS
 
 
 

EGFR Stably Expressing Cell Lines

 
Product   Principle   Data   Literature   Price/Order   Message
 

EGFR mutations occur in many type of cancers including non-small cell lung cancer. Common mutations found in these patients are deletions in exon 19 (Del19) and L858R substitution in exon 21.  Patients with these mutations are sensitive to first (gefitinib or erlotinib) and second (afatinib) generation EGFR tyrosine kinase inhibitors (TKIs) , whereas patients with wild type EGFR are not.  However, eventually all patients become resistant to first and/or second generation EGFR TKIs and acquired T790M secondary mutation in exon 20 accounts for more than 50% of the acquired resistance.  Cells expressing EGFR with either L858R+ T790M or Del19+T790M double mutations are resistant to induced apoptosis in the presence of first or second generation EGFR TKIs. 

 

Eight cell lines stably expressing HA-tagged EGFR have been established in the IL3-dependent Ba/F3 cell lines —WT EGFR, L858R EGFR mutant, L858R/T790M EGFR double mutant, Del19 (del747-752) EGFR mutant, Del19/T790M EGFR mutant, three ins20 (D770_N771insSVD,A763_Y764insFQEA, A767_dupASV) EGFR mutants —and Ba/F3 cell line expressing empty vector.  Also, two cell lines stably expressing HA-tagged EGFR have been established in HCC827 cell lines —Exon19del EGFR mutant, Exon19del/T790M EGFR double mutant—and HCC827 cell line expressing empty vector.

 

Due to their proliferation upon EGFR activation, sensitivity to TKIs, or resistance to induced apoptosis, these cell lines can be used to study the molecular mechanism underlying susceptibility of tumors to the drugs as well as screening and validating new TKIs.
 

 


Cell Lines Available: (Click for pricing and catalogue numbers)

 

Control (Empty vector) Ba/F3 Stable Cell Line EL-001
EGFR (wild-type) Stably Expressing Ba/F3 Cell Line EL-002
EGFR (L858R) Stably Expressing Ba/F3 Cell Line EL-003
EGFR (L858R+T790M) Stably Expressing Ba/F3 Cell Line EL-004
EGFR (E19del) Stably Expressing HCC827 Cell Line EL-005
EGFR (E19del+T790M) Stably Expressing HCC827 Cell Line EL-006
Control (Empty vector) HCC827 Cell Line EL-007      
EGFR( D770_N771insSVD )stably Expressing  BaF3 stable cell line  EL-008   
EGFR (A763_Y764insFQEA) stably Expressing BaF3 cells  EL-009
EGFR (A767_dupASV) stably  expressing  BaF3 cells  EL-010
EGFR ( DEL19) stably expressing BaF3 cells  EL-011
EGFR (Del19-T790M) stably expressing BaF3 stable cell line  EL-014





These cell lines are Suitable for the following applications: 
  
     

  1. - Research for Oncogenic tyrosine kinases
  2. - Research for lung cancer
  3. - Signal transduction
  4. - Research for EGF receptor and its downstream pathway
  5. - Screening for novel chemical inhibitors suing high-thoughput format.
  6. - Immunoprecipitation study
  7. - IF study




* Related Product you might be interested in: