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EMSA

 
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Transcription factors (TFs) are a group of cellular proteins that control gene expression. Once a TF is activated, it binds to its corresponding cis-element within promoter regions and mediates the expression of its target gene. Electrophoretic Mobility Shift Assay (EMSA) is a commonly used method for monitoring the activation of a TF in vitro. Each Signosis’ EMSA Kit is a ready-made kit including all of the reagents and transfer membranes.  The included probe has been designed based the reported TF consensus sequence in well-known publications and can be used to detect the activity of a TF in human, mouse and rat samples.

 

 
Benefits:
  • Isotope free It is a chemiluminescent-based detection.
  • No probe preparation The included probes are pre-labeled and optimized.
  • High Sensitive - With optimized conditions, The kit sensitivity is compatible with that from radioactive-based detection.
  • Simple procedure All of the reagents are included in the kit.


List of EMSA kits available:

GS-00

AHR EMSA Kit GS-0096 FOXA1 EMSA Kit GS-0049 MEF2 EMSA Kit GS-0024 Pbx1 EMSA Kit GS-0035 Sp1 EMSA Kit GS-0040
AP1 EMSA Kit GS-0001 FoxC1 EMSA Kit GS-0062 Myb EMSA Kit GS-0025 Pit EMSA Kit GS-0036 SRF EMSA Kit GS-0041
AP2 EMSA Kit GS-0002 FOXD3 EMSA Kit GS-0055 Myc-Max EMSA Kit GS-0026 PIT1 EMSA Kit GS-0068 SRY EMSA Kit GS-0074
AP3 EMSA Kit GS-0082 FOXG1 EMSA Kit GS-0076 MyoD EMSA Kit GS-0064 PLAG1 EMSA Kit GS-0079 Stat1 EMSA Kit GS-0043
AP4 EMSA Kit GS-0083 FOXO1 (FKHR) EMSA Kit GS-0075 MZF EMSA Kit GS-0086 PPAR EMSA Kit GS-0037 Stat3 EMSA Kit GS-0044
AR EMSA Kit  GS-0003 FREAC-2 (FOXF2) EMSA Kit GS-0095 NF-1 EMSA Kit GS-0027 PR EMSA Kit GS-0020 Stat4 EMSA Kit GS-0045
ATF2 EMSA Kit GS-0004 GAS/ISRE EMSA Kit GS-0017 NFAT EMSA Kit GS-0028 Prox1 EMSA Kit GS-0056 Stat5 EMSA Kit GS-0046
Brn-3 EMSA Kit GS-0005 GATA EMSA Kit GS-0018 NF-E2 EMSA Kit GS-0029 PXR EMSA Kit GS-0038 Stat6 EMSA Kit GS-0047
C/EBP EMSA Kit GS-0006 Gfi-1 EMSA Kit GS-0077 NFkB EMSA Kit GS-0030 RB Site EMSA Kit GS-0073 TCF/LEF EMSA Kit GS-0048
CAR EMSA Kit GS-0007 Gli EMSA Kit GS-0072  Nkx2-5 EMSA Kit GS-0062 RNUX EMSA Kit GS-0080 TFE3 EMSA Kit GS-0087
CBF EMSA Kit GS-0008 GR/PR EMSA Kit GS-0019 Nkx3-2 EMSA Kit GS-0066 ROR (RZR) EMSA Kit GS-0092 TFIID EMSA Kit GS-0049
CDP EMSA Kit GS-0009 HEN (NSCL-1) EMSA Kit GS-0094 NRF1 EMSA Kit GS-0091 RXR EMSA Kit GS-0081 TR EMSA Kit GS-0050
COUP-TF EMSA Kit GS-0084 HIF EMSA Kit GS-0021 NRF2 (ARE) EMSA Kit GS-0031 SATB1 EMSA Kit GS-0042 USF-1 EMSA Kit GS-0052
CREB EMSA Kit GS-0010 HNF-1 EMSA Kit GS-0093 OCT-1 EMSA Kit GS-0088 SMAD (MADH) EMSA Kit GS-0039 VDR EMSA Kit GS-0053
E2F-1 EMSA Kit GS-0011 HNF-4 EMSA Kit GS-0022 OCT-4 EMSA Kit GS-0032  SMUC EMSA Kit GS-0078 WT1 EMSA Kit GS-0071
EGR EMSA Kit GS-0012 HOX4C EMSA Kit GS-0085 p53 EMSA Kit GS-0033 Snail EMSA Kit GS-0089 YY1 EMSA Kit GS-0051
ELK EMSA Kit GS-0013 HOXA-5 EMSA Kit GS-0061 Pax2 EMSA Kit GS-0067 SOX18 EMSA Kit GS-0057 EMSA Kit w/o Probe GS-0000
ER EMSA Kit GS-0014 HSF EMSA Kit GS-0054 Pax3 EMSA Kit GS-0069 SOX2 EMSA Kit GS-0060 Combination EMSA Kit with Four Probes GS-1001
Ets EMSA Kit GS-0015 IRF EMSA Kit GS-0023 Pax5 EMSA Kit GS-0034 SOX5 EMSA Kit GS-0058  
FAST-1 (FOXH1) EMSA Kit GS-0016 KLF4 EMSA Kit GS-0090 Pax8 EMSA Kit GS-0070 SOX9 EMSA Kit GS-0059  


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