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FewCell TF Activation Profiling Plate Array

 
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 Benefits:
  • Designed for precious and limited cells - Requires as few as 2,000 cells.
  • Multiplex - One assay allows the measurement of the activities for 16 transcription factors.
  • One-step sample preparation - Obtain whole cell lysate with provided buffer. 
  • Quantitative comparison - Differences between samples can be quantitatively compared and analyzed.
  • Simple procedures - User friendly procedures similar to ELISA.
  • No special requirement - Capital instrument such as Luminex is not needed.
 

FewCell Stem Cell TF Activation Profiling Plate Array FA-1103


Stem cells are important cells characterized by their ability to self-renew or differentiate into many cell types, which is governed by intracellular signaling pathway and transcription regulation. Hence, transcription regulation plays a determining role in conferring cellular identity and function. The step-wise maturation of stem cells into terminally differentiated cell types requires the timely activation of a cascade of transcription programs governed by lineage-specifying transcription factors. Multiple transcription factors and target genes have been widely reported to associate with stem cell self-renewal and pluripotency including EGR1, OCT4, FOXD3, FOXO, Nanog, SOX2, SOX18, ETS, GLI, KLF4, MEF2, Myc, RNUX1, Pax6, TCF/LEF and GATA. However, stem cells are precious and limited.  Signosis has developed FewCell Stem Cell Transcription Factor Activation Plate Array to analyze activities of 16 stem cell-specific TFs simultaneously in mammalian samples.  The array requires as few as 2,000 cells and the cellular protein is extracted with one-step procedure using the whole cell lysis buffer. This kit contains one plate which can be used for 6 assays. 

List of Applicable TFs:

  1 2 3

4

5

6

7 8 9 10

11

12
A EGR1 Ets EGR1

Ets

EGR1

Ets

EGR1 Ets EGR1

Ets

EGR1

Ets
B FOXD3 Gli FOXD3 Gli FOXD3 Gli FOXD3 Gli FOXD3 Gli FOXD3 Gli
C FOXO1 KLF4

FOXO1

KLF4 FOXO1 KLF4 FOXO1 KLF4

FOXO1

KLF4 FOXO1 KLF4
D GATA MEF2 GATA MEF2 GATA MEF2 GATA MEF2 GATA MEF2 GATA MEF2
E Nanog Myc Nanog Myc Nanog Myc Nanog Myc Nanog Myc Nanog Myc
F OCT4 RNUX1 OCT4 RNUX1 OCT4 RNUX1 OCT4 RNUX1 OCT4 RNUX1 OCT4 RNUX1
G SOX2 PAX6 SOX2 PAX6 SOX2 PAX6 SOX2 PAX6 SOX2 PAX6

SOX2

PAX6
H SOX18 TCF/LEF SOX18 TCF/LEF SOX18 TCF/LEF SOX18 TCF/LEF SOX18 TCF/LEF SOX18 TCF/LEF
 
 Benefits:
  • Designed for precious and limited cells - Requires as few as 2,000 cells.
  • Multiplex - One assay allows the measurement of the activities for 16 transcription factors.
  • One-step sample preparation - Obtain whole cell lysate with provided buffer. 
  • Quantitative comparison - Differences between samples can be quantitatively compared and analyzed.
  • Simple procedures - User friendly procedures similar to ELISA.
  • No special requirement - Capital instrument such as Luminex is not needed.



FewCell ER Stress (UPR) TF Activation Profiling Plate Array FA-1106


The Unfolded Protein Response (UPR) is a conserved and essential stress response that cells activate to combat endoplasmic reticulum (ER) stress, commonly caused by the accumulation of misfolded proteins or failing protein quality control.  ER stress is a well-characterized feature of several diseases, such as diabetes, Alzheimer’s, Parkinson’s, Huntingon’s, and prion diseases.  Early cellular response to ER stress includes the transcriptional upregulation of chaperone proteins, which is mediated by a large number of transcription factors (TFs).  Signosis has developed the FewCell ER Stress/UPR TF Activation Profiling Plate Array, which can be used to simultaneously monitor 16 ER stress/UPR related TFs, including XBP-1, ATF4, ATF6, GADD153/CHOP, CBF/NFY, SREBP1, YY1, PGC-1a, ATF3, AP-1, FOXO1, IRF, p53, NFkB, NRF2/ARE, and HNF4. The array requires as few as 2,000 cells and the cellular protein is extracted with one-step procedure using the whole cell lysis buffer. This kit contains one plate which can be used for 6 assays.
 

List of Applicable TFs:

 

  1 2 3

4


5


6

7 8 9 10

11

12
A XBP-1 ATF3 XBP-1

ATF3


XBP-1


ATF3

XBP-1 ATF3 XBP-1

ATF3


XBP-1

ATF3
B ATF4 AP-1 ATF4 AP-1 ATF4 AP-1 ATF4 AP-1 ATF4 AP-1 ATF4 AP-1
C ATF6 FOXO1

ATF6

FOXO1 ATF6 FOXO1 ATF6 FOXO1

ATF6

FOXO1 ATF6 FOXO1
D GADD153 MEF2 GADD153 MEF2 GADD153 MEF2 GADD153 MEF2 GADD153 MEF2 GADD153 MEF2
E CBF/NFY p53 CBF/NFY p53 CBF/NFY p53 CBF/NFY p53 CBF/NFY p53 CBF/NFY p53
F SREBP1 NFkB SREBP1 NFkB SREBP1 NFkB SREBP1 NFkB SREBP1 NFkB SREBP1 NFkB
G YY1 NRF2/ARE YY1 NRF2/ARE YY1 NRF2/ARE YY1 NRF2/ARE YY1 NRF2/ARE

YY1

NRF2/ARE
H ERR HNF4 ERR HNF4 ERR HNF4 ERR HNF4 ERR HNF4 ERR HNF4
 
 Benefits:
  • Designed for precious and limited cells - Requires as few as 2,000 cells.
  • Multiplex - One assay allows the measurement of the activities for 16 transcription factors.
  • One-step sample preparation - Obtain whole cell lysate with provided buffer. 
  • Quantitative comparison - Differences between samples can be quantitatively compared and analyzed.
  • Simple procedures - User friendly procedures similar to ELISA.
  • No special requirement - Capital instrument such as Luminex is not needed.