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Kinase TAD Luciferase Reporter Stable Cell Line

 
Product   Principle   Data   Literature   Price/Order   Message
 

Kinases mediate cellular changes through the activation of transcription factors where the DNA binding activity of many transcription factors are regulated by phosphorylation.  Fusing the transactivation domain of a TF to the GAL4, this allows for specific activation of that TF by a kinase pathway.  Once phosphorylated, by direct or indirect phosphorylation, the fusion TAD-GAL4 will translocate to the nucleus and bind to UAS binding site to drive luciferase reporter expression.  Different from a promoter-reporter assay, the TAD offers greater specificity, whereas the promoter-based element may have multiple TFs and co-factors binding or may be activated by a kinase-independent method.  Signosis has created a stable cell lines that express TAD-GAL4 fusion protein and the UAS-luciferase reporter.  This tool can measure kinase activity, particularly in drug discovery, testing the function of an unknown kinase, or placing a kinase in a signaling pathway.


Benefits:


  • Routine mycoplasma testing - All cell lines tested negative for mycoplasma.
  • High sensitive and responsive - Each cell line is validated to induce strong reporter signal.
  • Consistent - Kinase-TAD reporter construct is stably integrated into the genome to avoid experimental/cell-to-cell variations.
  • Time saving - Cell line can be used for experiments right away to study different signaling pathways.
  • Firefly Luciferase Substrate -  Click to learn more about Signosis luciferase substrate.

 

 

Transcription Factors Pathway Cell Line
Academic  Industry
  • ELK
MAPK Signaling
HEK293; human embryonic kidney SL-0040-NP SL-0040-FP
ELK MAPK Signaling
Hela; human cervical cancer SL-0041-NP SL-0041-FP