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Kinase TAD Luciferase Reporter Vectors

 
Product   Principle   Data   Literature   Price/Order   Message
 

Kinases mediate cellular changes through the activation of transcription factors where the DNA binding activity of many transcription factors are regulated by phosphorylation.  Fusing the transactivation domain of a TF to the GAL4, this allows for specific activation of that TF by a kinase pathway.  Once phosphorylated, by direct or indirect phosphorylation, the fusion TAD-GAL4 will translocate to the nucleus and bind to UAS binding site to drive luciferase reporter expression.  Different from a promoter-reporter assay, the TAD offers greater specificity, whereas the promoter-based element may have multiple TFs and co-factors binding or may be activated by a kinase-independent method.  Signosis has created a single plasmid that contains the TAD-GAL4 fusion protein and the UAS-luciferase reporter to allow simple and efficient transfection.  This tool can measure kinase activity, particularly in drug discovery, testing the function of an unknown kinase, or placing a kinase in a signaling pathway. 



Benefits:


  • Two-in-one - Gal4-TAD fusion protein and UAS-luciferase reporter combined into a single vector.
  • Functional assay Monitor kinase activity in cells.
  • Quantitative analysis - Determine kinase activation by quantitative measurement of  luciferase activity.
  • Stable cell line generation The vectors can be used for establishing stable cells expressing the reporters.

 

 
List of available luciferase reporter vectors:

ATF2-TAD
LR-7001
cJUN-TAD
LR-7002
CREB-TAD
LR-7003
ELK-TAD
LR-7004
HIF-TAD
LR-7005
NRF2(113)-TAD
LR-7006
NRF2(370)-TAD
LR-7007
RelA(p65)-TAD
LR-7008
Stat3-TAD
LR-7009
Stat5b-TAD
LR-7010

 

List of kinase available:

Empty Vector
LR-7500
AKT (WT)
LR-7501
AKT (CA)
LR-7502
AKT (DN)
LR-7503
MEKK
LR-7504
MAPK1
LR-7505
ROCK1-N
LR-7506
ROCK1-C
LR-7507
PKA
LR-7508
MEK3
LR-7509
p38 MAPK (WT)
LR-7510
p38 MAPK (CA)
LR-7511
p38 MAPK (DN)
LR-7512
GSK3b
LR-7513
 

 

WT - Wild type

CA - Constitutive active

DN - Dominant negative

 

Firefly Luciferase SubstrateFor use with the reporter vectors.

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