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Promoter Binding TF Profiling Plate Array

 
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Benefits:
  • Multiplex - A single assay allows the characterization of binding of 48 or 96 TFs to a specific promoter.
  • Simple procedures - Probe incubation, spin column separation, plate hybridization, and HRP detection.
 

test


test only

 

Benefits:
  • Multiplex - A single assay allows the characterization of binding of 48 or 96 TFs to a specific promoter.
  • Simple procedures - Probe incubation, spin column separation, plate hybridization, and HRP detection.



Promoter Binding TF Profiling Plate Array I FA-2001


To characterize transcription factors (TFs) that binds to a specific promoter or that regulate the expression of a specific gene via its upstream promoter, two common approaches are applied. First is to employ gel shift assay with DNA binding sites of TFs that are silico-identified within the promoter. Second is to removal or knockout the binding site(s) of a specific TF in order to measure whether the expression of a promoter-linked reporter is increased or decreased. Often, a series of reporter constructs with the promoter deletions or mutations need to make because many binding sites of one or a few TFs are present within a promoter. Signosis has developed a fast method to facilitate the characterization of promoters through a revised TF activation array. This assay will help to test whether selected 48 or 96 TFs bind to the promoter or not.

 

List of Applicable TFs:

  1 2 3

4

5

6

7 8 9 10

11

12
A AP1 CDP GATA

NF-1

Pit

Stat3

AP1 CDP GATA

NF-1

Pit

Stat3
B AP2 CREB GR/PR NFAT PPAR Stat4 AP2 CREB GR/PR NFAT PPAR Stat4
C AR E2F-1

HIF

NF-E2 PXR Stat5 AR E2F-1

HIF

NF-E2 PXR Stat5
D ATF2 EGR HNF4 NFkB SMAD Stat6 ATF2 EGR HNF4 NFkB SMAD Stat6
E Brn-3 ER IRF OCT4 Sp1 TCF/LEF Brn-3 ER IRF OCT4 Sp1 TCF/LEF
F C\EBP Ets MEF2 p53 SRF TR C\EBP Ets MEF2 p53 SRF TR
G CAR FAST-1 Myb Pax-5 SATB1 YY1 CAR FAST-1 Myb Pax-5

SATB1

YY1
H CBF GAS/ISRE Myc-Max Pbx1 Stat1 TEIID CBF GAS/ISRE Myc-Max Pbx1 Stat1 TEIID

 

 

Benefits:
  • Multiplex - A single assay allows the characterization of binding of 48 or 96 TFs to a specific promoter.
  • Simple procedures - Probe incubation, spin column separation, plate hybridization, and HRP detection.



Promoter Binding TF Profiling Plate Array II FA-2002


To characterize transcription factors (TFs) that binds to a specific promoter or that regulate the expression of a specific gene via its upstream promoter, two common approaches are applied. First is to employ gel shift assay with DNA binding sites of TFs that are silico-identified within the promoter. Second is to removal or knockout the binding site(s) of a specific TF in order to measure whether the expression of a promoter-linked reporter is increased or decreased. Often, a series of reporter constructs with the promoter deletions or mutations need to make because many binding sites of one or a few TFs are present within a promoter. Signosis has developed a fast method to facilitate the characterization of promoters through a revised TF activation array. This assay will help to test whether a selected 96 TFs bind to the promoter or not.

List of Applicable TFs:

   1  2  3

 4

 5

 6

 7  8  9  10

 11

 12
A AP1 CDP GATA

NF-1

Pit

Stat3

XBP FOXG1 HoxA-5 NRF2(A)

ProX1

SOX2 
B AP2 CREB GR/PR NFAT PPAR Stat4  AP3  FOXO1  HSF Oct-1 RB SOX9 
C AR E2F-1

HIF

NF-E2 PXR Stat5 AP4  FREAC-2  KLF4 Pax2 RUNX SOX18
D ATF2 EGR HNF4 NFkB SMAD Stat6 COUP-TF  Gfi-1  MyoD Pax3 ROR(RZR) TFE3 
E Brn-3 ER IRF OCT4 Sp1 TCF/LEF ELK Gli-1 MZF Pax8 RXR TFEB
F C\EBP Ets MEF2 p53 SRF TR FOXA1 HEN (NSCL-1) Nkx2-5 PIT1 SF-1 USF-1
G CAR FAST-1 Myb Pax-5 SATB1 YY1 FOXC1 HNF-1 Nkx3-2 PLAG1

SMUC

VDR
H CBF GAS/ISRE Myc-Max Pbx1 Stat1 TFIID FOXD3  HOX4C NRF1 MEF1 Snail  WT1

 

 

Benefits:
  • Multiplex - A single assay allows the characterization of binding of 48 or 96 TFs to a specific promoter.
  • Simple procedures - Probe incubation, spin column separation, plate hybridization, and HRP detection.