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Signaling Protein Assay Kits

 
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SH2 Domain-Based RTK Profiling Plate Array DA-0001


Receptor tyrosine kinases (RTKs) play important roles in converting extracellular to intracellular signals.They are activated by the binding of specific growth factors, cytokines, or hormones, which results in autophosphorylation. Tyrosine autophosphorylation generates recruitment sites for downstream signaling proteins containing Src homology-2 (SH2). Binding of different SH2 domain-containing signaling proteins represents the activation of different signaling pathways. Therefore, the activation of signaling pathways can be assessed through examination of SH2 domain binding. Signosis provided SH2 Domain-Based RTK Profiling Plate Array, which allows assessing the activation of RTK signaling pathways through the examination of 46 SH2 domain binding simultaneously.

 
Benefits:
  • Multiplex - A single assay allows the measurement of the interaction of RTK with 46 SH2 domains.
  • Quantitative comparison - The difference of individual proteins in the interaction of the SH2 domains can be quantitatively compared and analyzed.
  • Simple - The assay is performed the same as the procedure of ELISA.

List of Arrayed SH2 Domains

  1 2 3 4 5 6 1 2 3 4 5 6
A 3BP2 FP-0001 CHN1 FP-0015 GRB10 FP-0030 LNK FP-0044 P1K3R1-D2 RaLP FP-0067 3BP2 FP-0001 CHN1 FP-0015 GRB10 FP-0030 LNK FP-0044 P1K3R1-D2 RaLP FP-0067
B ALX FP-0004 CHN2 FP-0016 GRB14 FP-0031 LYN FP-0045 P1K3R2-D2 RIN1 FP-0068 ALX FP-0004 CHN2 FP-0016 GRB14 FP-0031 LYN FP-0045 P1K3R2-D2 RIN1 FP-0068
C BCAR3 FP-0006 CRK FP-0018 GRB2 FP-0032 MATK FP-0046 PLCG1-D1 FP-0058 RASA1 BCAR3 FP-0006 CRK FP-0018 GRB2 FP-0032 MATK FP-0046 PLCG1-D1 FP-0058 RASA1
D BLK FP-0007 CRKL FP-0019 HCK FP-0036 MIST FP-0047 PLCG2-D2 FP-0061 SH2D2A BLK FP-0007 CRKL FP-0019 HCK FP-0036 MIST FP-0047 PLCG2-D2 FP-0061 SH2D2A
E BMX-3 FP-0009 CTEN FP-0021 ITK FP-0038 NCK1 FP-0048 PTPN6-D1 FP-0065 SH2D4A BMX-3 FP-0009 CTEN FP-0021 ITK FP-0038 NCK1 FP-0048 PTPN6-D1 FP-0065 SH2D4A
F BRDG1 FP-0010 FES FP-0025 JAK1 FP-0039 NCK2 FP-0049 PTPN6-D2 FP-0066 SHC FP-0078 BRDG1 FP-0010 FES FP-0025 JAK1 FP-0039 NCK2 FP-0049 PTPN6-D2 FP-0066 SHC FP-0078
G BTK FP-0011 FYN FP-0028 LCK FP-0042 NSP1 FP-0050 PTPNII-D1 FP-0063 GST BTK FP-0011 FYN FP-0028 LCK FP-0042 NSP1 FP-0050 PTPNII-D1 FP-0063 GST
H CBLC FP-0014 GRAP FP-0029 LCP2 FP-0043 P1K3R1-D1 PTPNII-D2 FP-0064 Blank CBLC FP-0014 GRAP FP-0029 LCP2 FP-0043 P1K3R1-D1 PTPNII-D2 FP-0064 Blank



Genome-Wide SH2-Domain GST Fusion Proteins


Src homology-2 (SH2) Domains are a segment of proteins that binds to phospho-tyrosine proteins. SH2 domain-containing proteins play important roles in mediating signaling transduction. There are total 120 SH2 domains in human genome. Identification of proteins that bind to a SH2 domain via GST fusion protein approach is crucial to dissecting signaling pathways. Genome-wide SH2 domain GST fusion proteins are available in Signosis, which can be used for binding analyses such as GST pull-down assays.

 
Benefits:

The proteins can be made at different amounts based upon inquiry.  The proteins on beads as well as GST fusion protein expression vectors are also made.


List of Applicable GST-SH2 Domains

3BP2 FP-0001 BTK FP-0011 CTEN FP-0021 GRB14 FP-0031 JAK3 FP-0041 NSP3 FP-0051 PLCG2-D2 FP-0061 RSG1-D1 FP-0071 SHD FP-0081 SOCS3 FP-0091 STAT3 FP-0101 TENS1 FP-0111
ABL1 FP-0002 CBL FP-0012 DAPP1 FP-0022 GRB2 FP-0032 LCK FP-0042 P55G-D1 FP-0052 PTK6 FP-0062 RSG1-D2 FP-0072 SHF FP-0082 SOCS4 FP-0092 STAT4 FP-0102 TNS FP-0112
ABL2 FP-0003 CBLB FP-0013 EAT2 FP-0023 GRB7 FP-0033 LCP2 FP-0043 P55G-D2 FP-0053 PTPN11-D1 FP-0063 SH1A FP-0073 SHIP FP-0083 SOCS5 FP-0093 STAT5A FP-0103 TXK FP-0113
ALX FP-0004 CBLC FP-0014 FER FP-0024 GRID FP-0034 LNK FP-0044 P85A-D1 FP-0054 PTPN11-D2 FP-0063 SH2A FP-0074 SHIP2 FP-0084 SOCS6 FP-0094 STAT5B FP-0104 TYK2 FP-0114
APS FP-0005 CHN1 FP-0015 FES FP-0025 GRP2 FP-0035 LYN FP-0045 P85A-D2 FP-00555 PTPN6-D1 FP-0065 SH2B FP-0075 SHP1L-D1 FP-0085 SOCS7 FP-0095 STAT6 FP-0105 VAV1 FP-0115
BCAR3 FP-0006 CHN2 FP-0016 FGR FP-0026 HCK FP-0036 MATK FP-0046 P85B-D1 FP-0056 PTPN6-D2 FP-0066 SH2D3C FP-0076 SHP1L-D2 FP-0086 SRC FP-0096 SUPT6H FP-0106 VAV2 FP-0116
BLK FP-0007 CISH FP-0017 FRK FP-0027 INPP5D FP-0037 MISTFP-0047 P85B-D2 FP-0057 RaLP FP-0067 SHB FP-0077 SLA1 FP-0087 SRMS FP-0097 SYK-D1 FP-0107 VAV3 FP-0117
BLNK FP-0008 CRK FP-0018 FYN FP-0028 ITK FP-0038 NCK1 FP-0048 PLCG1-D1 FP-0058 RIN1 FP-0068 SHC1 FP-0078 SLA2 FP-0088 STAP2 FP-0098 SYK-D2 FP-0108 YES FP-0118
BMX FP-0009 CRKL FP-0019 GRAP FP-0029 JAK1 FP-0039 NCK2 FP-0049 PLCG1-D2 FP-0059 RIN2 FP-0069 SHC2 FP-0079 SOCS1 FP-0089 STAT1 FP-0099 TEC FP-0109 ZAP70-D1 FP-0119
BRDG1 FP-0010 CSK FP-0020 GRB10 FP-0030 JAK2 FP-0040 NSP1 FP-0050 PLCG2-D1 FP-0060 RIN3 FP-0070 SHC3 FP-0080 SOCS2 FP-0090 STAT2 FP-0100 TENC1 FP-0110 ZAP70-D2 FP-0120