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Single Cell Gene Expression Analysis

 
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Single Cell Real-Time RT-PCR Assay Kit CL-0003


Gene expression is regulated by individual cells, so why not study gene expression in a single cell such as stem cells, neurons, developmental tissues, and laser capture microsections? To allow researchers the ability to take on single cell studies, Signosis has developed a single cell SYBR Green based real-time RT-PCR Assay kit which allows you to directly assay gene expression levels in a single cell using cell lysate without any RNA preparation. This kit provides a simple, sensitive, quantitative and cost-effective method to monitor PCR reactions in real time. The reagents for cDNA synthesis and PCR amplification in addition to Direct cDNA cell lysis buffer are also included in the kit.

 
Benefits:
  • All-in-One - All reagents for the experiment, from cell lysis, reverse transcription to PCR assay, are included in  one kit.
  • No need for RNA preparation - Cell lysates can be used directly in reverse transcription.
  • Robust - Full length cDNA can be obtained with the kit.
  • Real-time - Analysis can be monitored in real time.

 Single Cell PCR Single Cell Kit




Single Cell RT-PCR Assay Kit CL-0002


Gene expression is regulated at the level of individual cells. It is desired to have methods capable of analyzing the gene expression pattern of individual cells such as stem cells, neurons, developmental tissues, and laser capture microssections. Signosis has developed a sensitive single cell RT-PCR directly in cell lysates without RNA preparation. Therefore, a small number of cells can be used for RT-PCR analysis. Signosis now offers the optimized buffers for subsequent RT-PCR followed cell lysis with Direct cDNA cell lysis buffer. All of reagents for cDNA synthesis and PCR amplification in addition to Direct cDNA cell lysis buffer are included in the kit.




Direct cDNA Cell Lysis Buffer CL-0001


The study of gene expression often needs RNA preparation followed by cDNA synthesis and PCR, but most of the time, you don't want to waste a large amount of cells for RNA preparation. This is a particular problem for researchers using laser dissected samples, FACS sorted cells, and cultured cells in 96-wells. Signosis’ Direct cDNA cell lysis buffer allows researchers to prepare cell lysate from small samples, which can be used for direct reverse transcription without RNA preparation. Cell lysate made from even a few cells is good enough for reverse transcription of RNA to cDNA, which you can then use for PCR analysis or cDNA plate array assays.

 
Benefits:
  • Simplified procedures - Reverse transcription can be performed in cell lysate without RNA preparation.
  • Limited cell numbers required - A few cells are good enough to make cDNA for PCR.