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TF Luciferase Vector Sets

 
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Benefits:
    • Functional assay - Monitor the expression or transactivation of transcription factors in cells.
  • Quantitative analysis Quantitatively measure TF activation or expression via luciferase activity.
  • Comprehensive Monitor 7 transcription factors in one assay.


TF Luciferase Reporter Vector Sets Available:

pLuc5U-Oxidative Stress Luciferase Reporter Vector Set iNOS, Gadd153/CHOP, COX-2, HO-1, GPx-1, NRF2, BCL2 and Negative control
Oxidative Stress Luciferase Reporter Vector Set NRF2/ARE, HIF, NFkB, P53, HSF, AP1, Stat3 and Negative control
Apoptosis Luciferase Reporter Vector Set NFkB, p53, Myc, AP1, IRF, ELK, NFAT, and Negative control
Antioxidant Luciferase Reporter Vector Set NRF2/ARE, ElK-1, HIF, NFkB, p53, HSF, KLF2 and Negative control
Genotoxic Stress Luciferase Reporter Vector Set p53, NFkB, E2F1, CREB, ATF2, Myb, IRF, and Negative control
Inflammation Luciferase Reporter Vector Set NFkB, AP1, IRF, CREB, Stat3, C/EBP, RUNX1, and Negative control
Hypoxic Response Luciferase Reporter Vector HIF, AP1, p53, ElK, SP1, NFkB, ATF4, and Negative control

UPR/ER Stress Luciferase Reporter Vector AP1, NFkB, ATF6, NRF2/ARE, XBP1, CHOP, SREBP1, and Negative control

 

Firefly Luciferase SubstrateFor use with the reporter vectors.

 

UPR/ER Stress Luciferase Reporter Vector Set


The Unfolded Protein Response (UPR) is a conserved and essential stress response that cells activate to combat endoplasmic reticulum (ER) stress, commonly caused by the accumulation of misfolded proteins or failing protein quality control.  ER stress is a well-characterized feature of several diseases, such as diabetes, Alzheimer’s, Parkinson’s, Huntingon’s, and prion diseases.  Early cellular response to ER stress includes the transcriptional upregulation of chaperone proteins, which is mediated by a large number of transcription factors (TFs).

 


Signosis has developed a set of luciferase reporters (AP1, NFkB, NRF2/ARE, ATF6, XBP1, CHOP, SREBP1 and Negative control) to facilitate UPR/ER stress study.

 

Sets Available: (Click below for pricing and ordering)

UPR/ER Stress Luciferase Reporter Vector Set (50ng/vector) LR-3007-50
UPR/ER Stress Luciferase Reporter Vector Set (2000ng/vector) LR-3007-2000

Benefits:
    • Functional assay - Monitor the expression or transactivation of transcription factors in cells.
  • Quantitative analysis Quantitatively measure TF activation or expression via luciferase activity.
  • Comprehensive Monitor 7 transcription factors in one assay.



pLuc5U-Oxidative Stress Luciferase Reporter Vector Set


The excessive production of reactive oxidative species (ROS) leads to a condition of oxidative stress. Oxidative stress can directly damage cell structures and may results in cancer, inflammatory diseases, and aging. iNOS, Gadd153/CHOP, COX-2, HO-1, GPx-1, NRF2, and BCL2 are important inducible mediators during the oxidative stress process. The measurement of the changes in transcriptional regulation of these genes is vital to monitoring the oxidative stress status and deciphering the underlying mechanisms of this critical cellular function in response to a wide range of cellular stresses. Signosis has developed a set of gene promoter luciferase reporters for these genes including iNOS, Gadd153/CHOP, COX-2, HO-1, GPx-1, NRF2, BCL2, and Negative Control. These vectors contain the reporter luciferase gene downstream of the selected promoter region of each gene, which functional testing has been well documented.

 

Sets Available:(Click below for pricing and ordering)

pLuc5U-Oxidative Stress Luciferase Reporter Vector Set (50ng/vector) LR-5001-50
pLuc5U-Oxidative Stress Luciferase Reporter Vector Set (2000ng/vector) LR-5001-2000

Benefits:
    • Functional assay - Monitor the expression or transactivation of transcription factors in cells.
  • Quantitative analysis Quantitatively measure TF activation or expression via luciferase activity.
  • Comprehensive Monitor 7 transcription factors in one assay.



Oxidative Stress Luciferase Reporter Vector Set


Oxidative stress is caused by an imbalance in the generation and removal of reactive oxygen species (ROS).  ROS elicits a wide spectrum of responses, such as cell proliferation, growth arrest, or apoptosis. ROS comes mostly from the endogenous metabolic by-products and also from exogenous sources such as exposure to cigarette smoke, environmental pollutants, and bacterial, fungal or viral infections. Oxidative damage has been implicated in the cause of many diseases, such as cancer, Alzheimer's disease, Parkinson's disease, atherosclerosis, and heart failure. A number of signaling pathways are involved in coordinating the response to elevations in ROS. One critical change in signaling pathways is the activation of transcription factors (TFs), and TF-based reporters are often used to monitor the activation of signaling pathways.  

Signosis has developed a set of luciferase reporters (NRF2/ARE, HIF, NFkB, p53, HSF, AP1, Stat3 and Negative control) to facilitate oxidative stress study. 

 

Sets Available:(Click below for pricing and ordering)

Oxidative Stress Luciferase Reporter Vector Set (50ng/vector) LR-3001-50
Oxidative Stress Luciferase Reporter Vector Set (2000ng/vector) LR-3001-2000

Benefits:
    • Functional assay - Monitor the expression or transactivation of transcription factors in cells.
  • Quantitative analysis Quantitatively measure TF activation or expression via luciferase activity.
  • Comprehensive Monitor 7 transcription factors in one assay.



Apoptosis Luciferase Reporter Vector Set


Apoptosis is known as programmed cell death implicated in biological processes ranging from embryogenesis to ageing and from normal tissue homoeostasis to many human diseases. A number of signaling pathways that lead to characteristic cell changes are involved in mediating the apoptosis responses. One critical change in signaling pathways is the activation of transcription factors (TFs), and TF-based reporters are often used to monitor the activation of signaling pathways. 

Signosis has developed a set of luciferase reporter vectors (NFkB, p53, Myc, AP1, IRF, ELK, NFAT, and Negative control) to facilitate apoptosis study.

 

Sets Available:(Click below for pricing and ordering)

Apoptosis Luciferase Reporter Vector Set (50ng/vector) LR-3002-50
Apoptosis Luciferase Reporter Vector Set (2000ng/vector) LR-3002-2000

Benefits:
    • Functional assay - Monitor the expression or transactivation of transcription factors in cells.
  • Quantitative analysis Quantitatively measure TF activation or expression via luciferase activity.
  • Comprehensive Monitor 7 transcription factors in one assay.



Antioxidant Luciferase Reporter Vector Set


Reactive oxygen species (ROS) are critical to cell proliferation, growth arrest, and apoptosis. ROS comes mostly from the endogenous metabolic by-products and also from exogenous sources such as exposure to cigarette smoke, environmental pollutants, and bacterial, fungal or viral infections. Excess ROS must be promptly eliminated from the cell by different antioxidant defense mechanisms, in order to reduce oxidative that has been implicated in the cause of many diseases, such as cancer, Alzheimer's disease, Parkinson's disease, atherosclerosis, and heart failure. Cellular antioxidant enzymes and other redox molecules serve to counterbalance ROS generated in the cell. A number of signaling pathways are involved in coordinating antioxidant responses to ROS elevation. One critical change in signaling pathways is the activation of transcription factors (TFs), and TF-based reporter vectors are often used to monitor the activation of signaling pathways. 

Signosis has developed a set of luciferase reporter vectors (NRF2/ARE, ELK-1, HIF, NFkB, P53, HSF, KLF2 and Negative control) to facilitate antioxidant study. 

 

Sets Available:(Click below for pricing and ordering)

Antioxidant Luciferase Reporter Vector Set (50ng/vector) LR-3003-50
Antioxidant Luciferase Reporter Vector Set (2000ng/vector) LR-3003-2000

Benefits:
    • Functional assay - Monitor the expression or transactivation of transcription factors in cells.
  • Quantitative analysis Quantitatively measure TF activation or expression via luciferase activity.
  • Comprehensive Monitor 7 transcription factors in one assay.



Genotoxic Stress Luciferase Reporter Vector Set


Exposure to genotoxic agents such as ultraviolet light, oxidative stress, and chemical mutagens leads to DNA damage, which is a major cause of human cancers. Cellular responses are important to effective defense against genotoxic stress.  These responses affect many cellular factors, which form an extensive signal transduction network, and the activation of transcription factors (TF), which in turn regulate the expression of genes involved in DNA repair, cell cycle arrest and apoptosis. TF-based reporter vectors are often used to monitor the activation of signaling pathways. 

Signosis has developed a set of luciferase reporter vectors (p53, NFkB, E2F1, CREB, GLI, Myb, IRF, and Negative control) to facilitate genotoix stress study. 

 

Sets Available:(Click below for pricing and ordering)

Genotoxic Stress Luciferase Reporter Vector Set (50ng/vector) LR-3004-50
Genotoxic Stress Luciferase Reporter Vector Set (2000ng/vector) LR-3004-2000

Benefits:
    • Functional assay - Monitor the expression or transactivation of transcription factors in cells.
  • Quantitative analysis Quantitatively measure TF activation or expression via luciferase activity.
  • Comprehensive Monitor 7 transcription factors in one assay.



Inflammation Luciferase Reporter Vector Set


Inflammation is a complex physiological reaction to noxious stimuli including tissue stress, injury and infection. Dysregulation of inflammation often occurs in people and can cause a variety of pathologies, ranging from chronic inflammation, to autoimmunity, to cancer. What leads to the inducible expression of inflammatory genes at the level of gene transcription is a crucial question, and the answer lies in transcription factors (TF).  TF-based reporters are often used to monitor the activation of signaling pathways. 

Signosis has developed a set of luciferase reporter vectors (NFkB, AP1, IRF, CREB, Stat3, C/EBP, RUNX1, and Negative control) to facilitate inflammation study. 

 

Sets Available:(Click below for pricing and ordering)

Inflammation Luciferase Reporter Vector Set (50ng/vector) LR-3005-50
Inflammation Luciferase Reporter Vector Set (2000ng/vector) LR-3005-2000

Benefits:
    • Functional assay - Monitor the expression or transactivation of transcription factors in cells.
  • Quantitative analysis Quantitatively measure TF activation or expression via luciferase activity.
  • Comprehensive Monitor 7 transcription factors in one assay.



Hypoxic Response Luciferase Reporter Vector Set


Cells induce the hypoxia responses to adapt to the environment when organisms are exposed to a low oxygen environment. Hypoxic areas are a common feature of rapidly growing malignant tumors and their metastases. Tissue hypoxia due to inadequate blood supply is supposed to occur very early during tumor development. Hypoxic response includes the activation of certain signal transduction pathways and gene regulatory mechanisms, tumor cell apoptosis and tumor angiogenesis. What leads to the inducible expression of inflammatory genes at the level of gene transcription is a crucial question, and the answer lies in transcription factors (TF).  TF-based reporters are often used to monitor the activation of signaling pathways. 

Signosis has developed a set of luciferase reporter vectors (HIF, AP1, p53, ElK, SP1, NFkB, ATF4, and Negative control) to facilitate hypoxic response study. 

 

Sets Available:(Click below for pricing and ordering)

Hypoxic Response Luciferase Reporter Vector Set (50ng/vector) LR-3006-50
Hypoxic Response Luciferase Reporter Vector Set (2000ng/vector) LR-3006-2000

Benefits:
    • Functional assay - Monitor the expression or transactivation of transcription factors in cells.
  • Quantitative analysis Quantitatively measure TF activation or expression via luciferase activity.
  • Comprehensive Monitor 7 transcription factors in one assay.