PRODUCTS   METHODS
 
 
 

TF Protein Interaction Plate Array I

 
Product   Principle   Data   Literature   Price/Order   Message
 
Click for 
Price Information and 

Catalogue Numbers 
 
Benefits:
  • Multiplex - One assay allows the measurement of the transcriptional interaction of various TFs with a TF or co-activator of interest.
  • Quantitative comparison - Differences between samples can be quantitatively compared and analyzed.
  • Simple procedures - Co-IP of TF complexes with spin column is quick, easy, and effective.
  • No special equipment required - Compatible with common chemiluminescence plate readers.
 

TF Protein Interaction Plate Array I FA-3001


Transcription factors (TFs) are a group of cellular proteins that play essential roles in regulating gene expression. They act as sensors to monitor cellular change and convert the signals into gene expression. Often, TFs regulate gene expression not only through directly binding to their DNA binding site on the target promoter region, but also by  interacting with other transcription factors or regulators. Since changes in transcriptional interaction patterns can have dramatic effects on gene expression and cellular responses to stimuli, monitoring multiple TF/TF and TF/co-regulator interactions simultaneously could yield a greater understanding of regulatory mechanisms. Signosis’ TF Protein Interaction Plate Array I can monitor TF/TF or TF/co-regulator interactions among 48 different TFs simultaneously in relation to one TF or co-regulator of interest.


List of Applicable TFs:

  1 2 3

4

5

6

7 8 9 10

11

12
A AP1 CDP GATA

NF-1

Pit

Stat3

AP1 CDP GATA

NF-1

Pit

Stat3
B AP2 CREB GR/PR NFAT PPAR Stat4 AP2 CREB GR/PR NFAT PPAR Stat4
C AR E2F-1

HIF

NF-E2 PXR Stat5 AR E2F-1

HIF

NF-E2 PXR Stat5
D ATF2 EGR HNF4 NFkB SMAD Stat6 ATF2 EGR HNF4 NFkB SMAD Stat6
E Brn-3 ER IRF OCT4 Sp1 TCF/LEF Brn-3 ER IRF OCT4 Sp1 TCF/LEF
F C\EBP Ets MEF2 p53 SRF TR C\EBP Ets MEF2 p53 SRF TR
G CAR FAST-1 Myb Pax-5 SATB1 YY1 CAR FAST-1 Myb Pax-5

SATB1

YY1
H CBF GAS/ISRE Myc-Max Pbx1 Stat1 TFIID CBF GAS/ISRE Myc-Max Pbx1 Stat1 TEIID
 
Benefits:
  • Multiplex - One assay allows the measurement of the transcriptional interaction of various TFs with a TF or co-activator of interest.
  • Quantitative comparison - Differences between samples can be quantitatively compared and analyzed.
  • Simple procedures - Co-IP of TF complexes with spin column is quick, easy, and effective.
  • No special equipment required - Compatible with common chemiluminescence plate readers.