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cDNA Plate Arrays from Cell Lysates

 
Product   Principle   Data   Literature   Price/Order   Message
 
 
Benefits:
  • Simple DNA can be synthesized  directly in cell lysates without RNA preparation.  In addition, the detection is as simple as ELISA.
  • Quantitative comparison Detected mRNA molecules are converted into multiple biotin-labeled cDNAs for sensitive detection.
  • Quantitative comparison  The difference of two or more samples in gene expression can be quantitatively analyzed. 
 

Human NFkB-Regulated cDNA Profiling in Cell Lysates AP-3101


NFkB is a ubiquitous transcription factor that plays a key role in cellular responses to stimuli such as stress, cytokines, free radicals, ultraviolet irradiation, oxidized LDL, and bacterial or viral antigens. Incorrect regulation of NFkB has been linked to cancer, inflammatory and autoimmune diseases, septic shock, viral infection, and improper immune development. When NFkB is activated, it is dissociated from its inhibitor IkB and moves from the cytoplasm to the nucleus, where it binds to target DNA elements and positively regulates the transcription of genes involved in immune and inflammatory responses, cell growth control, and apoptosis. To get a better overview of gene expression of NFkB regulated genes, Signosis has developed a cDNA Plate Array which couples its CLTM cDNA synthesis kit which allows reverse transcription directly from cell lysates without RNA preparation. This plate-based assays allows profiling the expression of 20+ NFkB-regulated genes directly in cell lysates. This assay provides quantitative data, allowing researchers to screen multiple samples, as well as high sensitivity, allowing researchers to use less sample. 

List of Applicable Genes

BCL2 IL6  Myc  BCL2 IL6  Myc  BCL2 IL6  Myc  BCL2 IL6  Myc 
Beta-actin  IL8  NOS2  Beta-actin  IL8  NOS2  Beta-actin  IL8  NOS2  Beta-actin  IL8  NOS2 
CCND1 GAPDH  p53 CCND1 GAPDH  p53 CCND1 GAPDH  p53 CCND1 GAPDH  p53
Cox-2  IFNg TNFA  Cox-2  IFNg TNFA  Cox-2  IFNg TNFA  Cox-2  IFNg TNFA 
FAS-L  IRF1  TNFR  FAS-L  IRF1  TNFR  FAS-L  IRF1  TNFR  FAS-L  IRF1  TNFR 
IFNB1  MDR1 VCAM1 IFNB1  MDR1 VCAM1 IFNB1  MDR1 VCAM1 IFNB1  MDR1 VCAM1
IL1A  MMP1  VEGFC IL1A  MMP1  VEGFC IL1A  MMP1  VEGFC IL1A  MMP1  VEGFC
IL2 Myb Blank IL2 Myb Blank IL2 Myb Blank IL2 Myb Blank

NFkB cDNA

 
Benefits:
  • Simple DNA can be synthesized  directly in cell lysates without RNA preparation.  In addition, the detection is as simple as ELISA.
  • Quantitative comparison Detected mRNA molecules are converted into multiple biotin-labeled cDNAs for sensitive detection.
  • Quantitative comparison  The difference of two or more samples in gene expression can be quantitatively analyzed. 



Human HIF-Regulated cDNA Profiling in Cell Lysates AP-3102


Rapidly growing tumors result in hypoxic regions. Adaptive responses of most cells to hypoxia are (1) to produce VEGF and other hypoxia-induced angiogenic cytokines that promote increased tissue vascularization, thereby increasing tissue oxygenation, and (2) to switch metabolically from oxidative phosphorylation to anaerobic glycolysis. Hypoxia-inducible factor 1 (HIF-1) is an oxygen-regulated transcriptional activator that plays essential roles in the process. The HIF-1alpha subunit is oxygen-dependent ubiquitination and proteasomal degradation. In addition, cytokines including interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha) stimulate HIF-1 dependent gene expression. HIF-1 increases the expression of several genes that promote blood flow and inflammation, such as vascular endothelial growth factor (VEGF). To get a better overview of gene expression of HIF-1 regulated genes, Signosis has developed a cDNA Plate Array which couples its CLTM cDNA synthesis kit which allows reverse transcription directly from cell lysates without RNA preparation. This plate-based assays allows profiling the expression of 20+ HIF-1-regulated genes directly in cell lysates. This assay provides quantitative data, allowing researchers to screen multiple samples, as well as high sensitivity, allowing researchers to use less sample. 

List of Applicable Genes

BCL2 HIF-2a PDGF-A BCL2 HIF-2a PDGF-A BCL2 HIF-2a PDGF-A BCL2 HIF-2a PDGF-A
Beta-actin HPRT PDK1 Beta-actin HPRT PDK1 Beta-actin HPRT PDK1 Beta-actin HPRT PDK1
CA9 IGF-II NDRG1 CA9 IGF-II NDRG1 CA9 IGF-II NDRG1 CA9 IGF-II NDRG1
CTSD  Leptin SAG CTSD  Leptin SAG CTSD  Leptin SAG CTSD  Leptin SAG
BHLHB2 MMP1 SLC6A8 BHLHB2 MMP1 SLC6A8 BHLHB2 MMP1 SLC6A8 BHLHB2 MMP1 SLC6A8
Glut-1 MXI-1 VEGF Glut-1 MXI-1 VEGF Glut-1 MXI-1 VEGF Glut-1 MXI-1 VEGF
Glut-3 NDRG2 VHL Glut-3 NDRG2 VHL Glut-3 NDRG2 VHL Glut-3 NDRG2 VHL
HIF-1a PAI-1 Blank HIF-1a PAI-1 Blank HIF-1a PAI-1 Blank HIF-1a PAI-1 Blank
 
Benefits:
  • Simple DNA can be synthesized  directly in cell lysates without RNA preparation.  In addition, the detection is as simple as ELISA.
  • Quantitative comparison Detected mRNA molecules are converted into multiple biotin-labeled cDNAs for sensitive detection.
  • Quantitative comparison  The difference of two or more samples in gene expression can be quantitatively analyzed.