IFN-α/ISRE Responsive Luciferase Reporter HeLa Cell Line is derived human cervical cancer, and stably express firefly luciferase reporter gene under the control of IFN-α/ISRE response element. This cell line is an ideal cellular model for monitoring the activation of TYK2 and JAK1 response Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
 
Interferon-alpha (IFN-α) binds to receptor subunits IFNαR1 and IFNαR2 on the cell surface, which initiate the phosphorylation of TYK2 and JAK1 and trigger downstream cascade of JAK-Stat signal transduction pathways. IFN-α can also induce the formation of Interferon-stimulated gene factor 3 (ISGF3), composed of STAT1, STAT2, and IRF9. The ISGF3 complexes bind ISRE (IFN-stimulated response elements) further inducing the transcription of IFN-stimulated genes which contain ISREs within their promoters that leads to transcription of selected genes. Signosis developed the IFN-α/ISRE luciferase reporter stable cell line to monitor IFN-α associated JAK-Stat pathway.
 
Principle behind TF luciferase reporter. TF luciferase reporter stable cell line utilizes artificial promoter constructs to drive luciferase expression. The promoter region can consists of multiple repeats of a cis-element TF binding site, a DNA fragment from the promoter region of a known TF downstream gene, or a DNA fragment containing putative/known TF binding sites. There are several ways that a TF can be activated, such as through extracellular stimuli or through intracellular signaling pathways. Once activated, the TF translocates to the nucleus and often interacts with relevant co-factors to drive gene expression. Once luciferase is expressed, it can generate light in an enzymatic assay and the amount of light measured is positively correlated with the level of TF activation.