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miRNA Luciferase Reporter Vectors

 
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Benefits:
  • Functional assay Monitor miRNA expression in cells.
  • Quantitative analysis - Expression and activation of miRNAs are measured through suppression of firefly luciferase gene expression.
 

miRNA Luciferase Reporter Vectors


pMiR-Luc reporter vectors are a series of firefly luciferase-based reporter constructs for quantitative measurement of miRNA expression in cells. Each vector contains the CMV promoter, firefly luciferase gene, a unique miRNA target site at 3’UTR, and a SV40 terminator sequence. The target site is a sequence perfectly complementary to a specific miRNA. When the miRNA is expressed, it binds to the sequence and results in repression of luciferase gene expression. Therefore, luciferase activity represents the expression and activity of a miRNA.

List of miRNA Luciferase Reporter Vectors available:

pLet7a-Luc LR-0037 pLet7b-Luc LR-0001 pLet7c-Luc LR-0038 pLet7d-Luc LR-0039 pLet7e-Luc LR-0040 pLet7f-Luc LR-0041 pLet7g-Luc LR-0042 pLet7i-Luc LR-0043
pMiR-1-Luc LR-0044 pMiR-7-5p-Luc LR-0002 pMiR-7b-5p-Luc LR-0003 pMiR-9-5p-Luc LR-0054 pMiR-10a-5p-Luc LR-0045 pMiR-10b-5p-Luc LR-0046 pMiR-15a-5p-Luc LR-0004 pMiR-15b-5p-Luc LR-0049
pMiR-16-5p-Luc LR-0005 pMiR-17-5p-Luc LR-0006 pMiR-17-3p-Luc LR-0007 pMiR-18a-5p-Luc LR-0051 pMiR-18b-5p-Luc LR-0062 pMiR-19a-3p-Luc LR-0008 pMiR-19b-3p-Luc LR-0053 pMiR-19-5p-Luc LR-0087
pMiR-20a-5p-Luc LR-0009 pMiR-21-5p-Luc LR-0010 pMiR-24-3p-Luc LR-0011 pMiR-26a-5p-Luc LR-0012 pMiR-27a-Luc LR-0088 pMiR-28-Luc LR-0075 pMiR-29a-3p-Luc LR-0013 pMiR-29b-3p-Luc LR-0063
pMiR-29c-3p LR-0074 pMiR-30b-Luc LR-0089 pMiR-34a-5p-Luc LR-0014 pMiR-34b-5p-Luc LR-0057 pMiR-34c-5p-Luc LR-0058 pMiR-99a-Luc LR-0092 pMiR-101-3p-Luc LR-0015 pMiR-101a-Luc LR-0085
pMiR-106a-5p-Luc LR-0016 pMiR-106b-3p-Luc LR-0061 pMiR-107-Luc LR-0017 pMiR-122-Luc LR-0076 pMiR-122a-5p-Luc LR-0018 pMiR-125a-5p-Luc LR-0019 pMiR-125b-5p-Luc LR-0020 pMiR-127-5p-Luc LR-0021
pMiR-132-3p-Luc LR-0022 pMiR-141-Luc LR-0077 pMiR-144-3p-Luc Lr-0023 pMiR-145-5p-Luc LR-0048 pMiR-146a-5p-Luc LR-0024 pMiR-155-5p-Luc Lr-0025 pMiR-153-3p LR-0064 pMiR-181-Luc LR-0084
pMiR-181a-5p-Luc LR-0026 pMiR-181b-5p-Luc Lr-0050 pMiR-194-5p-Luc LR-0027 pMiR-195-5p LR-0065 pMiR-196a-5p-Luc LR-0028 pMiR-198-Luc LR-0078 pMiR-199a-5p-Luc LR-0029 pMiR-200-Luc LR-0079
pMiR-200a-Luc LR-0086 pMiR-200c-3p LR-0073 pMiR-205-5p-Luc LR-0055 pMiR-206-Luc LR-0030 pMiR-208a-3p-Luc LR-0056 pMiR-208b-3p LR-0072 pMiR-210-3p-Luc LR-0059 pMiR-214-3p-Luc LR-0031
pMiR-221-3p-Luc LR-0032 pMiR-222-3p-Luc LR-0033 pMiR-223-3p LR-0066 pMiR-320-Luc LR-0080 pMiR-349-Luc LR-0081 pMiR-372-3p-Luc LR-0035 pMiR-373-5p-Luc Lr-0036 pMiR-380-Luc LR-0090
(mum)pMiR-380-5p-Luc LR-0067 pMiR-448 LR-0069 pMiR-451-Luc LR-0082 pMiR-451a-Luc LR-0060 pMiR-455-5p LR-0071 pMiR-496a-3p LR-0068 pMiR-655-Luc LR-0083 pMiR-885-Luc LR-0091
pMiR-885-5p LR-0070 pMiR-Luc-Control LR-1000  pMiR-148a-Luc LR0092          

 

Benefits:
  • Functional assay Monitor miRNA expression in cells.
  • Quantitative analysis - Expression and activation of miRNAs are measured through suppression of firefly luciferase gene expression.



miRNA Target Reporter Vectors


MicroRNAs regulate gene expression by targeting messenger RNAs at specific sites to induce the cleavage of the targets or inhibit translation. The interaction of a miRNA with its targets is known mediated by RNA-induced silencing complex (RISC). RISC-mediated interaction between a mature miRNA and its binding site depends on sequence complementarities between miRNA and its targets and the total number of binding sites in a given 3’UTR. Numerous important regulatory genes have been found to be regulated by miRNAs, such as Ras by let-7, Bcl-2 by miR-15 and miR-16, ERa by miR206, TPM1 by miR-21, and PTEN by miR-19a. Luciferase reporter-based cellular assays are often employed to monitor the regulation of these genes by miRNAs.

 

List of miRNA Target Reporter Vectors available:

pLuc3U-BCL2 LR-1001 pLuc3U-E2F3 LR-1005 pLuc3U-HNF1A LR-1009 pLuc3U-RB LR-1013 pLuc3U-Twist LR-1017
pLuc3U-Cox2 LR-1002 pLuc3U-EGFR LR-1006 pLuc3U-HOXD10 LR-1010 pLuc3U-TCL1 LR-1014  
pLuc3U-DNMT3B LR-1003 pLuc3U-ERa LR-1007 pLuc3U-KRAS LR-1011 pLuc3U-TPM1 LR-1015  
pLuc3U-E2F1 LR-1004 pLuc3U-Fos LR-1008 pLuc3U-PTEN LR-1012 pLuc3U-TSP1 LR-1016  




 

Benefits:
  • Functional assay Monitor miRNA expression in cells.
  • Quantitative analysis - Expression and activation of miRNAs are measured through suppression of firefly luciferase gene expression.