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miRNA Real Time PCR

 
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Benefits:
  • Convenient All reagents, from cell lysis to PCR, are included in one kit.
  • No RNA preparation Cell lysates can be used directly in reverse transcription, although total RNA is perfectly fine.
  • Real-time Analysis can be monitored in real time.
 

miRNA Real-Time PCR Assay Kit CL-0004


miRNAs are known to involve in multiple biological functions and abnormal expression is associated to human diseases such as cancers. They represent a promising new class of cancer biomarkers. A number of PCR methods have been developed for analyzing the expression level of miRNA. Based on its proprietary technology, Signosis has developed a highly sensitive and discriminative real-time PCR assay method for measuring miRNA expression. It implements oligo-ligation and SYBR green based real-time PCR. The assay can be used for quantitative analysis of miRNA expression in either total RNA or cell lysate without cDNA conversion.

 

List of Applicable miRNAs: 

let-7a-5p PO-0001 miR-22-3p PO-0021 miR-99a-5p PO-0041 miR-135b-5p PO-0061 miR-181a-5p PO-0081 miR-198 PO-0101 miR-223-3p PO-0121 miR-720 PO-0141
let-7b-5p PO-0002 miR-23a-3p PO-0022 miR-100-5p PO-0042 miR-136-5p PO-0062 miR-181b-5p PO-0082 miR-199a-5p PO-0102 miR-224-5p PO-0122 miR-760 PO-0142
let-7c-5p PO-0003 miR-24-3p PO-0023 miR-101-3p PO-0043 miR-137 PO-0063 miR-181c-5p PO-0083 miR-199a*-3p PO-0103 miR-296-5p PO-0123  
let-7d-5p PO-0004 miR-25-3p PO-0024 miR-103-3p PO-0044 miR-140-5p PO-0064 miR-181d-5p PO-0084 miR-199b-5p PO-0104 miR-329 PO-0124  
let-7e-5p PO-0005 miR-26a-5p PO-0025 miR-106a-5p PO-0045 miR-141-3p PO-0065 miR-182-5p PO-0085 miR-200a-3p PO-0105 miR-337-3p PO-0125  
let-7f-5p PO-0006 miR-27a-3p PO-0026 miR-106b-5p PO-0046 miR-142-3p PO-0066 miR-183-5p PO-0086 miR-200c-3p PO-00106 miR-338 PO-0126  
let-7g-5p PO-0007 miR-28-5p PO-0027 miR-107 PO-0047 miR-142-5p PO-0067 miR-184 PO-0087 miR-202-5p PO-0107 miR-342-3p PO-00127  
let-7i-5p PO-0008 miR-29a-3p PO-0028 miR-122a-5p PO-0048 miR-143-3p PO-0068 miR-185-5p PO-0088 miR-203a PO-0108 miR-345 PO-0128  
miR-1 PO-0009 miR-29b-3p PO-0029 miR-124a-3p PO-0049 miR-144 PO-0069 miR-186-5p PO-0089 miR-204-5p PO-0109 mir-376c-3p (miR-368 PO-0129)
 
miR-7-5p PO-0010 miR-29c-3p PO-0030 miR-125a-5p PO-0050 miR-145-5p PO-0070 miR-188-5p PO-0080 miR-205-5p PO-0100 miR-369-3p PO-0110  
miR-9-5p PO-0011 miR-30a-3p PO-0031 miR-125b-5p PO-0051 miR-146a-5p PO-0071 miR-190-5p PO-0091 miR-206 PO-0111 miR-369-5p PO-0131  
miR-10a-5p PO-0012 miR-30a-5p PO-0032 miR-126-3p PO-0052 miR-146b-5p PO-0072 miR-191-5p PO-0092 miR-210-3p PO-0112 miR-371 PO-00132  
miR-15a-5p PO-0013 miR-30b-5p PO-0033 miR-127 PO-0053 miR-148a-3p PO-0073 miR-192-5p PO-0093 miR-213 (miR-181a) PO-0113 miR-372-3p PO-00133  
miR-16-5p PO-0014 miR-30c-5p PO-0034 miR-128a-3p PO-0054 miR-149-5p PO-0074 miR-193a-3p PO-0094 miR-214-3p PO-0114 miR-373-5p PO-0134  
miR-17-3p PO-0015 miR-31-5p PO-0035 miR-128b-3p PO-0055 miR-150-5p PO-0075 miR-193a-5p PO-0095 miR-215-5p PO-0115 miR-375 PO-0135  
miR-17-5p PO-0016 miR-92-3p PO-0036 miR-131 PO-0056 miR-151-3p PO-0076 miR-194-5p PO-0096 miR-216-5p PO-0116 miR-488-5p PO-0136  
miR-18a-5p PO-0017 miR-92b-3p PO-0037 miR-132-3p PO-0057 miR-153-3p PO-0077 miR-195-5p PO-0097 miR-218-5p PO-0117 miR-497 PO-0137  
miR-19a-3p PO-0018 miR-93-5p PO-0038 miR-133a-3p PO-0058 miR-152 PO-0078 miR-196a-5p PO-0098 miR-219 PO-0118 miR-499-5p PO-0138  
miR-20a-5p PO-0019 miR-95-3p PO-0039 miR-133b PO-0059 miR-154-5p PO-0079 miR-196b-5p PO-0099 miR-221-3p PO-0119 miR-507 PO-0139  
miR-21-5p PO-0020 miR-96 PO-0040 miR-134-5p PO-0060 miR-155-5p PO-0080 miR-197-3p PO-0100 miR-222-3p PO-0120 miR-609 PO-0140  

 

Benefits:
  • Convenient All reagents, from cell lysis to PCR, are included in one kit.
  • No RNA preparation Cell lysates can be used directly in reverse transcription, although total RNA is perfectly fine.
  • Real-time Analysis can be monitored in real time.



miR-17-92 Cluster Real-Time PCR Assay Kit CL-0005


The miR-17-92 cluster encoding miR-17-3p, miR-17-5p, miR-18a, miR-19a, miR-19b, miR--20a and miR-92 is highly expressed in tumor cells and are known to act as oncogenes. Expression of these miRNAs promotes cell proliferation, suppresses apoptosis of cancer cells, and induces tumor angiogenesis. New work reveals essential functions for these miRNAs not only in tumor formation but also during normal development of the heart, lungs, and immune system. A number of PCR methods have been developed for analyzing the expression level of miRNA. Based on its proprietary technology, Signosis has developed a highly sensitive and discriminative real-time PCR assay method for measuring multiple miRNA expression simultaneously, including miR-17-3P, miR-17-5p, miR-18a, miR-19a, miR-19b, miR-20a, miR-92. It implements oligo-ligation and SYBR green based real-time PCR. The assay can be used for quantitative analysis of miRNA expression in either total RNA or cell lysate without cDNA conversion.

 

Benefits:
  • Convenient All reagents, from cell lysis to PCR, are included in one kit.
  • No RNA preparation Cell lysates can be used directly in reverse transcription, although total RNA is perfectly fine.
  • Real-time Analysis can be monitored in real time.



Let7 Isoform Real-Time PCR CL-0006


Let7 family is known to regulate proliferation and differentiation processes in species ranging from C- elegans to human. The mature forms (designated let-7a through let-7i ) differ at one to four positions from the let-7a sequence. The expression level of the isoforms is variable, which may play a diverse role in organ development and disease etiology. To distinguish these isoforms will help to elicit the mechanism of Let7 function. Based on its proprietary technology, Signosis has developed a highly sensitive and discriminative real-time PCR assay method for measuring miRNA expression of let7 isoforms (Let7a, Let7b, Let7c, Let7d, Let7e, Let7f, Let7g and Let7i) simultaneously. The assay implements oligo-ligation and SYBR green based real-time PCR, which can be used for quantitative analysis of miRNA expression in either total RNA or cell lysate without cDNA conversion.

 

Benefits:
  • Convenient All reagents, from cell lysis to PCR, are included in one kit.
  • No RNA preparation Cell lysates can be used directly in reverse transcription, although total RNA is perfectly fine.
  • Real-time Analysis can be monitored in real time.