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STAT3 is activated in response to various cytokines and growth factors including IL-6 and OSM. The activation of STAT3 results in formation of STAT3 homodimers and STAT3/STAT1 heterodimers that translocate to the cell nucleus and induce transcription of genes by binding to the consensus element on the promoter region of target genes associated with cell growth and apoptosis. Signosis has established STAT3 luciferase reporter HEK293 stable cell line, which can be used as a reporter system for monitoring the activation of Stat3 triggered by stimuli treatment, enforced gene expression and gene knockdown. 

The cell line was established by transfection using a pTA-Stat3-luciferase reporter vector, which contains 4 repeats of Stat3 binding sites, a minimal promoter upstream of the firefly luciferase coding region, along with hygromycin expression vector followed by hygromycin selection. The hygromycin resistant clones were subsequently screened for oncostatin induced luciferase activity. The clone with the highest fold induction (10 fold) was selected and expanded to produce this stable cell line.



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