JAK/STAT3
Principle
STAT3 is a critical transcription factor that is activated in response to various cytokines and growth factors such as IL-6 and oncostatin M (OSM). The activation of STAT3 results in formation of STAT3 homodimers and STAT3/STAT1 heterodimers that translocate to the cell nucleus and induce transcription of genes by binding to the consensus element on the promoter region of target genes associated with cell growth and apoptosis.
To simplify the study of these pathways, Signosis has created STAT3 luciferase reporter stable cell lines that provide a sensitive and convenient tool for monitoring STAT3 activation. These cell lines can be applied to evaluate pathway responses to various stimuli, assess the impact of gene overexpression or knockdown, and screen drug candidates targeting the JAK/STAT signaling cascade.
These stable cell lines were established by transfection using a pTA-Stat3-luciferase reporter vector, which contains 4 repeats of Stat3 binding sites, a minimal promoter upstream of the firefly luciferase coding region, along with hygromycin expression vector followed by hygromycin selection. The hygromycin resistant clones were subsequently screened for oncostatin induced luciferase activity. The clone with the highest fold induction (10 fold) was selected and expanded to produce this stable cell line.
