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miRNA Plate Assays

The miRNA Plate Assay enables scientists to directly analyze miRNA without the need for cDNA conversion. This assay is 100 times more sensitive than the traditional miRNA Northern Blot Assay and is capable of discriminating between different miRNA isoform sequences, ensuring precise analysis of miRNA expression.

Principle 

The miRNA Plate Assay allows for the direct analysis of miRNA without the need for cDNA conversion. In this assay, the target miRNA molecule is flanked by a capture oligo and a biotinylated detection oligo, bridged by two additional oligos. One bridge oligo is partially hybridized with the miRNA and the capture oligo, while the other is hybridized with both the miRNA and the detection oligo.

This hybridization is immobilized onto a plate through the interaction with an immobilized oligo and detected using streptavidin-HRP conjugate and a chemiluminescent substrate.

Unlike the Northern Blot Assay, the miRNA Plate Assay is highly sensitive and can detect even small sequence differences between miRNA isoforms. A single nucleotide difference will block hybrid formation, enabling precise differentiation of miRNA isoforms. Additionally, this assay offers 100 times more sensitivity than the traditional Northern Blot, making it ideal for detecting low-abundance miRNAs.

miRNA plate assay diagram (single).gif

Products

Assay
Product
SKU
Price (USD$)
Plate Assay
MA-0102
655
Plate Assay
MA-0104
586
Plate Assay
MO-XXXX
545

Benefits

Convenience

Simplified procedure does not require the conversion of miRNA into cDNA.

High Sensitivity

miRNA is measured using chemiluminescent detection, which is 100 times more sensitive than the miRNA Northern Blot Assay.

Differentiate miRNA Isoforms

Can distinguish single nucleotide differences in miRNA isoforms.

Quantitative Comparison

The difference between two or more samples can be analyzed quantitatively.

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