miRNAs are known to involve in multiple biological functions and abnormal expression is associated to human diseases such as cancers. They represent a promising new class of cancer biomarkers. A number of multiplex methods such as microarray or microbeads assays are commonly used for profiling the expression of multiple miRNAs simultaneously. However these methods need the conversion of the miRNA into a cDNA probe during analysis. Signosis has developed a simple miRNA direct hybridization plate array method, in which total RNA does not need any pre-treatment before hybridization. It is as simple as direct hybridization and detection. More importantly, total 48 miRNAs can be quantitatively analyzed and compared simultaneously with the sample assay.