Luciferase is an enzyme that catalyzes a bioluminescent reaction, producing visible light as a byproduct. This enzyme is naturally found in various organisms, including certain insects, fungi, and marine animals, with the firefly being one of the most iconic examples. Notably, luciferase is absent in mammalian cells, making it an excellent reporter enzyme for biological research. Because luciferase activity is highly sensitive, has a wide dynamic range of quantification, and lacks of...