Signosis’ TF Activation Profiling Arrays are used for monitoring the activities of multiple TFs simultaneously. In the technology, a series of biotin-labeled probes are made based on the consensus sequences of TF DNA-binding sites. When the probe mixtures incubate with nuclear extracts, individual probes will find their corresponding TFs and form TF/probe complexes, which can be easily separated from free probes through a simple spin column purification.

 

The bound probes are then detached from the complexes and analyzed through hybridization with a plate, which each well is specifically pre-coated with complementary sequences of the probes. 

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TF Activation Plates comprise three distinct categories: Few Cell TF, TF Activation Plate, and Functional TF. The TF Activation Plate enables the simultaneous screening of a minimum of 48 and a maximum of 192 transcription factors. Functional TFs facilitate the screening of transcription factors associated with specific pathways of interest, such as ER Stress and Cholesterol, among others. Few Cell TF allows the screening of transcription factors using a restricted quantity of cells.