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Cas9-U6/H1 DualGuide
Single-Vector Knockout Systems
Signosis offers a comprehensive suite of Cas9-U6/H1 DualGuide Single-Vector Knockout Systems, featuring a single-vector design with dual-promoter sgRNA expression for each knockout target, optimized for enhanced knockout efficiency and targeting pathway regulators, kinases, and receptors with increased reliability
Principle
The CRISPR-Cas9 All-in-One Knockout Vectors offer a complete, ready-to-use solution for precise gene disruption across a broad range of cellular systems. Featuring a one-vector dual sgRNA design, this system streamlines the CRISPR editing workflow, enhancing both efficiency and consistency. By integrating Cas9 and two sgRNAs targeting distinct regions of the same gene or locus into a single vector, this approach simplifies cloning, reduces transfection complexity, and ensures higher co-delivery rates. The result is more reliable and efficient gene knockouts, making it ideal for gene function studies, pathway analysis, and therapeutic target validation.
Signosis offers a suite of Cas9-U6/H1 DualGuide Single-Vector Knockout Systems targets key regulators in major signal transduction pathways We can also customize our vectors to target your specific gene or pathway of interest, providing tailored solutions for your research needs.
Key features include:
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Dual-sgRNA, single-vector design: Integrates Cas9 and dual sgRNA expression cassettes into a single vector, simplifying cloning and reducing DNA load. This design eliminates the need for multiple vectors, improving workflow efficiency and up to 30-50% higher knockout efficiency compared to traditional pooled systems. It’s especially beneficial for difficult-to-transfect cells like primary or suspension cells, where co-delivery is often challenging.
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Dual sgRNA expression: Independent U6 and H1 promoters drive the expression of two sgRNAs targeting distinct regions within the same gene or locus. This minimizes promoter interference and ensures balanced sgRNA expression, increasing the probability of complete gene disruption and improving knockout efficiency by 15-25% compared to single-promoter or pooled vector systems.
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Enhanced editing efficiency: Co-expression of two sgRNAs targeting different regions within a gene improves editing precision, boosts the likelihood of bi-allelic knockouts, and reduces escape variants. Typical knockout efficiencies range from 60-80%, ensuring robust gene editing outcomes.
Benefits
All-in-One Design
Cas9 and two sgRNAs in a single vector
Reliable Dual Targeting
Disrupts multiple regions of the same gene
Dual Promoter Expression
U6 and H1 minimize interference for balanced sgRNA levels
Easy Transfection
One vector simplifies delivery and reduces variability
Higher Knockout Efficiency
Up to 20–30% improvement over pooled sgRNA vectors
Customizable
sgRNAs can be tailored to your gene of interest
Products
Target | Name | SKU | Price (USD$) |
|---|---|---|---|
NRF2/ARE | NRF2 KO Vector | KO-0001 | $800.00 |
NRF2/ARE | Keap1 KO Vector | KO-0002 | $800.00 |
NFkB | NFKBIA (IκBα) KO Vector | KO-0003 | $800.00 |
NFkB | p65 KO Vector | KO-0004 | $800.00 |
p53 | MDM2 KO Vector | KO-0005 | $800.00 |
p53 | p53 KO Vector | KO-0006 | $800.00 |
ER Stress-ERSE | PERK/EIF2AK3 KO Vector | KO-0007 | $800.00 |
ER Stress-ERSE | IRE1/ERN1 KO Vector | KO-0008 | $800.00 |
ER Stress-ERSE | ATF6 KO Vector | KO-0009 | $800.00 |
SMAD/BMP | Smad1/5/8 KO Vectors | KO-0010 | $800.00 |
SMAD/BMP | BMPR1 KO Vector | KO-0011 | $800.00 |
SMAD/BMP | BMPR2 KO Vector | KO-0012 | $800.00 |
SMAD/BMP | Smad4 KO Vector | KO-0013 | $800.00 |
SMAD/TGFbeta | TGFBR2 KO Vector | KO-0014 | $800.00 |
SMAD/TGFbeta | Smad2/3 KO Vectors | KO-0015 | $800.00 |
SMAD/TGFbeta | Smad7 KO Vectors | KO-0016 | $800.00 |
TCF/LEF | β-catenin KO Vector | KO-0017 | $800.00 |
TCF/LEF | AXIN1 KO Vector | KO-0018 | $800.00 |
Control | FLUC Positive Control KO Vector | KO-C001 | $800.00 |
Control | GLUC Positive Control KO Vector | KO-C002 | $800.00 |
Control | Non-Targetting Negative Control KO Vector | KO-C003 | $800.00 |
Control | hAASV1 Negative Control KO Vector | KO-C004 | $800.00 |
null | Custom KO Vector | cus-KO-0001 | $900.00 |