Cas9 DualGuide KO Vectors
Signosis offers a comprehensive suite of Cas9 DualGuide KO Vectors, featuring a single-vector design with dual-promoter sgRNA expression for each knockout target, optimized for enhanced knockout efficiency and targeting pathway regulators, kinases, and receptors with increased reliability
Principle
Signosis offers our Cas9 DualGuide KO Vectors: a complete, ready-to-use solution for precise gene disruption across a broad range of cellular systems—no cloning, no design, no CRISPR expertise required. Just transfect and go.
Featuring a dual-sgRNA design driven by independent U6 and H1 promoters, this all-in-one vector ensures robust co-expression of Cas9 and two guide RNAs. This dual-sgRNA design enhances editing performance, boosting knockout efficiency, ensuring bi-allelic gene disruption, and reducing escape variants compared to single-guide or multi-vector systems. Studies show dual-sgRNA systems routinely achieve ≥ 60% editing in diverse cell types.
Optimized for pathway-focused research, our vectors support investigations into cell signaling pathways—without any CRISPR background.
Key features include:
-
All‑in‑one Cas9 + dual‑sgRNA plasmid: Simplifies delivery and ensures high co-expression—ideal for hard-to-transfect cells.
-
Independent U6 + H1 promoter control: Minimizes promoter interference and balances sgRNA expression for robust knockout.
-
High editing efficiency: Dual-guide strategy promotes ≥ 60% knockout rates and efficient bi-allelic disruption.
-
Pathway discovery, simplified: Prebuilt vectors targeting key regulators, with easy customization—just transfect and go.
-
Plug and Play: Pair with our TF Reporter Cell Lines for easy functional readouts.
-
Customization Targeting: Need to target a specific gene or pathway? We’ll help design and build a vector tailored to your research.
Benefits
All-in-One Design
Cas9 and two sgRNAs in a single vector
Reliable Dual Targeting
Disrupts multiple regions of the same gene
Dual Promoter Expression
U6 and H1 minimize interference for balanced sgRNA levels
Easy Transfection
One vector simplifies delivery and reduces variability
Higher Knockout Efficiency
Up to 20–30% improvement over pooled sgRNA vectors
Customizable
sgRNAs can be tailored to your gene of interest
Products
Target | Name | SKU | Price (USD$) |
|---|---|---|---|
NRF2/ARE | NRF2 KO Vector | KO-0001 | $800.00 |
NRF2/ARE | Keap1 KO Vector | KO-0002 | $800.00 |
NFkB | NFKBIA (IκBα) KO Vector | KO-0003 | $800.00 |
NFkB | p65 KO Vector | KO-0004 | $800.00 |
p53 | MDM2 KO Vector | KO-0005 | $800.00 |
p53 | p53 KO Vector | KO-0006 | $800.00 |
ER Stress-ERSE | PERK/EIF2AK3 KO Vector | KO-0007 | $800.00 |
ER Stress-ERSE | IRE1/ERN1 KO Vector | KO-0008 | $800.00 |
ER Stress-ERSE | ATF6 KO Vector | KO-0009 | $800.00 |
SMAD/BMP | Smad1/5/8 KO Vectors | KO-0010 | $800.00 |
SMAD/BMP | BMPR1 KO Vector | KO-0011 | $800.00 |
SMAD/BMP | BMPR2 KO Vector | KO-0012 | $800.00 |
SMAD/BMP | Smad4 KO Vector | KO-0013 | $800.00 |
SMAD/TGFbeta | TGFBR2 KO Vector | KO-0014 | $800.00 |
SMAD/TGFbeta | Smad2/3 KO Vectors | KO-0015 | $800.00 |
SMAD/TGFbeta | Smad7 KO Vectors | KO-0016 | $800.00 |
TCF/LEF | β-catenin KO Vector | KO-0017 | $800.00 |
TCF/LEF | AXIN1 KO Vector | KO-0018 | $800.00 |
Control | FLUC Positive Control KO Vector | KO-C001 | $800.00 |
Control | GLUC Positive Control KO Vector | KO-C002 | $800.00 |
Control | Non-Targetting Negative Control KO Vector | KO-C003 | $800.00 |
Control | hAASV1 Negative Control KO Vector | KO-C004 | $800.00 |
null | Custom KO Vector | cus-KO-0001 | $900.00 |