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TF ELISA Kits
Signosis’ highly sensitive Transcription Factor (TF) ELISA Kits provide a reliable and efficient method for detecting low levels of activated transcription factors in nuclear extracts. Compared to traditional techniques like EMSA or supershift assays, these kits offer a faster, easier workflow without compromising sensitivity—making them ideal for routine screening and high-throughput applications.
Principle
The Signosis TF ELISA Kit is designed to detect activated transcription factors in nuclear extract or whole cell lysate using a plate-based, antibody-mediated detection system. The 96-well (12×8) strip plate is pre-coated with a double-stranded DNA oligonucleotide containing the consensus binding sequence specific to the target transcription factor (TF).
When the sample is added, activated TFs bind to the immobilized DNA. A primary antibody specific to the TF subunit is then applied to detect the bound TF, followed by an HRP-conjugated secondary antibody. Upon addition of TMB substrate, a colorimetric signal is generated, which is directly proportional to the amount of activated TF in the sample. The signal is measured using a standard microplate reader, providing a sensitive and quantitative assessment of TF activation.

TF | Product | SKU | Price (USD$) |
---|---|---|---|
NFkB p65 | TE-0001 | 828 | |
NFkB p50 | TE-0002 | 828 | |
IRF3 | TE-0005 | 828 | |
IRF7 | TE-0006 | 828 | |
IRF3, IRF7 | TE-0007 | 828 | |
SATB1 | TE-0008 | 828 | |
PPARγ | TE-0009 | 828 | |
Smad2/3 | TE-0011 | 828 | |
Smad1/5/8 | TE-0013 | 828 | |
Smad2/3, Smad1/5/8 | TE-0015 | 828 | |
FOXA1 | TE-0019 | 828 | |
STAT3 | TE-0020 | 828 | |
STAT1 | TE-0023 | 828 | |
STAT1, STAT3 | TE-0025 | 828 | |
NRF2 | TE-0027 | 828 | |
OCT4 | TE-0031 | 828 | |
SOX2 | TE-0033 | 828 | |
OCT4, SOX2 | TE-0035 | 828 | |
ATF4, ATF6 | TE-0037 | 828 | |
ATF4 | TE-0039 | 828 | |
ATF6 | TE-0041 | 828 |
Products
Benefits
Positive Control
An active recombinant protein with DNA binding activity is included in the kit as a positive control.
Singleplex and Duplex Formats
Can detect up to two TFs with one ELISA kit.
Highly Specific
Determines the activation of a specific TF gene using its DNA binding sequence and a specific antibody against the TF.
High-Throughput
Cell lysates from a 96-wel tissue culture plate can be prepared with Signosis' Whole Cell Lysis Buffer and analyzed with the TF ELISA kit.