TF ELISA Kits

Our highly sensitive TF ELISA kits can detect low levels of activated transcription factors in nuclear extracts. Unlike other methods such as electrophoretic mobility shift assays (EMSA) and supershift, TF ELISA kits are user-friendly and less time-consuming.

Principle

The TF ELISA kit utilizes a 96-well (12x8) strip plate that is pre-immobilized with the TF consensus sequencing oligo. Nuclear extract or whole cell lysate is added to the wells and the activated TF in the sample binds to the oligo. The activated TF is then detected with a specific antibody against the subunit and a HRP-conjugated secondary antibody. To determine the TF levels in the samples, TMB substrate is added to the wells for a colorimetric readout that can be measured with a microplate reader.

Benefits

Positive Control

An active recombinant protein with DNA binding activity is included in the kit as a positive control.

Singleplex and Duplex Formats

Can detect up to two TFs with one ELISA kit.

Highly Specific

Determines the activation of a specific TF gene using its DNA binding sequence and a specific antibody against the TF.

High-Throughput

Cell lysates from a 96-wel tissue culture plate can be prepared with Signosis' Whole Cell Lysis Buffer and analyzed with the TF ELISA kit.

TF	Product	SKU	Price (USD$)
NFkB p65	NFkB p65 ELISA Kit (Colorimetric)	TE-0001	828
NFkB p50	NFkB p50 ELISA Kit (Colorimetric)	TE-0002	828
IRF3	IRF3 ELISA Kit (Colorimetric)	TE-0005	828
IRF7	IRF7 ELISA Kit (Colorimetric)	TE-0006	828
IRF3, IRF7	IRF3/IRF7 ELISA Kit (Colorimetric)	TE-0007	828
SATB1	SATB1 ELISA Kit (Colorimetric)	TE-0008	828
PPARγ	PPARγ gamma ELISA Kit (Colorimetric)	TE-0009	828
Smad2/3	Smad2/3 ELISA Kit (Colorimetric)	TE-0011	828
Smad1/5/8	Smad1/5/8 ELISA Kit (Colorimetric)	TE-0013	828
Smad2/3, Smad1/5/8	Smad2/3 and Smad1/5/8 ELISA Kit (Colorimetric)	TE-0015	828
FOXA1	FOXA1 ELISA Kit (Colorimetric)	TE-0019	828
STAT3	STAT3 ELISA Kit (Colorimetric)	TE-0020	828
STAT1	STAT1 ELISA Kit (Colorimetric)	TE-0023	828
STAT1, STAT3	STAT1/STAT3 ELISA Kit (Colorimetric)	TE-0025	828
NRF2	NRF2 ELISA Kit (Colorimetric)	TE-0027	828
OCT4	OCT4 ELISA Kit (Colorimetric)	TE-0031	828
SOX2	SOX2 ELISA Kit (Colorimetric)	TE-0033	828
OCT4, SOX2	OCT4/SOX2 ELISA Kit (Colorimetric)	TE-0035	828
ATF4, ATF6	ATF4/ATF6 ELISA Kit (Colorimetric)	TE-0037	828
ATF4	ATF4 ELISA Kit (Colorimetric)	TE-0039	828
ATF6	ATF6 ELISA Kit (Colorimetric)	TE-0041	828

Quick Lead Time | Global Overnight Delivery Available

Purchase Order: info@signosisinc.com

TF ELISA Kits

Principle

Benefits

Products